| ~（131）I标记肝癌单克隆抗体HAb18F（ab＇）_2的NBS法研究 |
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| 引用本文： | 边惠洁,陈志南,何凤昌,米力,刘成刚. ~（131）I标记肝癌单克隆抗体HAb18F（ab＇）_2的NBS法研究[J]. 细胞与分子免疫学杂志, 1996, 0(2) |
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| 作者姓名： | 边惠洁 陈志南 何凤昌 米力 刘成刚 |
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| 作者单位： | 第四军医大学病理学教研室国家８６３肝癌导向药物课题组，新疆军区２３医院消化科 |
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| 摘 要： | 本文研究了以溴代琥珀酰亚胺（ＮＢＳ）为氧化剂的（１３１）Ⅰ标记肝癌单克隆抗体ＨＡｂ１８Ｆ（ａｂ’）２的方法。探讨了反应体系中ＮＢＳ的用量，Ｆ（ａｈ’）２的用量及Ｎａ（１３１）Ⅰ活度三个因素对标记率的影响，并从反应机理上予以解释。
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| 关 键 词： | 抗体片段；核素标记；碘－131；溴代琥珀酰亚胺 |
A N-bromosuccinimide Oxidant Method for Labeling F (ab')_2 Fragments of McAb HAb18 by Radioiodine-131 |
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| Bian Huijie, Chen Zhinan,He Fengchang, Mi Li, Liu Chenggang. A N-bromosuccinimide Oxidant Method for Labeling F (ab')_2 Fragments of McAb HAb18 by Radioiodine-131[J]. Chinese journal of cellular and molecular immunology, 1996, 0(2) |
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| Authors: | Bian Huijie Chen Zhinan He Fengchang Mi Li Liu Chenggang |
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| Abstract: | A N-bromosuccinimide (NBS) oxidant method for labeling F (ah' )2 fragments ofMcAb HAb18 by radioiodine-131 was described. The effects of NBS concentration, F (ah' )2concentration and Na131I activity on labeling efficiency were studied. |
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| Keywords: | McAb fragment isotope labeling iodine-131 j N-bromosuccinimide |
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