野西瓜果皮中有机酸类化学成分的分离与鉴定

目的研究野西瓜(Capparis spinosa L.)果皮中有机酸类化学成分,为进一步阐明其抗炎抗菌活性成分、充分开发利用提供科学依据。方法采用硅胶柱色谱、羟丙基葡聚糖凝胶柱色谱等方法进行分离纯化,并通过理化常数测定和波谱分析鉴定其化学结构。结果从野西瓜果皮中分离得到了6个有机酸类化合物,即香草酸(vanillic acid,1)、4-羟基苯甲酸(4-hydroxybenzoic acid,2)、原儿茶酸(protocatechuic acid,3)、2,4-二羟基苯甲酸(2,4-dihydroxybenzoic acid,4)、丁二酸(succinic acid,5)、邻苯二甲酸二(2-乙基)己酯(bis(2-ethylhexyl)phthalate,6);分离得到3个甾体类化合物为β-谷甾醇(β-sitosterol,7)、胡萝卜苷(daucosterol,8)、α-菠菜甾醇-3-O-β-D-吡喃葡萄糖苷(α-spinasterol-3-O-β-D-glucopyranoside,9)。结论化合物4、6、9为首次从山柑属植物中分离得到。

Isolation and identification of organic acids from pericarp of Capparis spinosa L.

Abstract：Objective To study the organic acids from the peel of Capparis spinosa. Methods The compounds were isolated by column chromatography and identified on the basis of physico-chemical constants and spectral analysis. Results Six organic acids (1-6) and three steroids (7-9) were isolated, their structures were identified as vanillic acid (1)，4-hydroxybenzoic acid (2), protocatechuic acid (3), 2, 4-dihydroxybenzoic acid (4), succinic acid (5), bis(2-ethylhexyl)phthalate (6), β-sitosterol (7), daucosterol (8) and α-spinasterol-3-O-β-D-glucopyranoside (9), respectively. Conclusion Compounds 4, 6, 9 are obtained from Capparis genus for the first time.