| 放线共生放线杆菌培养上清对成骨细胞分化及间隙连接通讯的影响 |
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| 引用本文： | 陈允嘉,王豫蓉,邹林洪,李闻颖,郑雷蕾,吴艳,李艳.放线共生放线杆菌培养上清对成骨细胞分化及间隙连接通讯的影响[J].第三军医大学学报,2010,32(10). |
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| 作者姓名： | 陈允嘉 王豫蓉 邹林洪 李闻颖 郑雷蕾 吴艳 李艳 |
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| 作者单位： | 重庆医科大学附属口腔医院正畸科,重庆,400015 |
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| 摘 要： | 目的探讨放线共生放线杆菌(actinobacillus actinomycetemcom itans,A.a)培养上清对成骨细胞分化以及细胞间隙连接通讯的影响。方法在A.a培养上清中体外培养成骨细胞,中性红检测成骨细胞活性,RT-PCR检测成骨细胞分化标记,免疫组化观察Ⅰ型胶原蛋白表达,利用划痕标记荧光染料示踪(SLDT)法与Westernblot检测Connexin43的表达,研究成骨细胞间隙连接通讯的变化。结果20%以下浓度的A.a培养上清对成骨细胞无明显致死性。RT-PCR结果显示A.a培养上清中成骨细胞分化标记的表达降低。免疫组化结果显示A.a培养上清中成骨细胞Ⅰ型胶原蛋白表达降低。SLDT法结果显示A.a培养上清中成骨细胞荧光染料扩散低于对照组。Western blot检测结果显示A.a培养上清中成骨细胞Connexin43表达降低。结论A.a培养上清对成骨细胞分化及细胞间隙连接通讯有抑制作用。
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| 关 键 词： | 放线共生放线杆菌 成骨细胞 分化 细胞间隙连接通讯 |
Actinobacillus actinomycetemcomitans induces differentiation and gap junctional intercellular communication in cultured osteoblasts |
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| Chen Yunjia,Wang Yurong,Zou Linhong,Li Wenying,Zheng Leilei,Wu Yan,Li Yan.Actinobacillus actinomycetemcomitans induces differentiation and gap junctional intercellular communication in cultured osteoblasts[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(10). |
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| Authors: | Chen Yunjia Wang Yurong Zou Linhong Li Wenying Zheng Leilei Wu Yan Li Yan |
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| Institution: | Chen Yunjia,Wang Yurong,Zou Linhong,Li Wenying,Zheng Leilei,Wu Yan,Li Yan (Department of Orthodontics,Affiliated Hospital of Stomatology,Chongqing Medical University,Chongqing,400015,China) |
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| Abstract: | Objective To investigate the effect of the supernatant of Actinobacillus actinomycetemcomitans (A.a) on the differentiation and gap junctional intercellular communication in osteoblasts in vitro.Methods Osteoblastic cells were maintained in BHI growth medium mixed with A.a culture supernatants.Neutral red was used to test cellular activity.Quantitative real-time PCR assay was used to detect the differentiation markers,including alkaline phosphatase (ALP),osteocalcin (OCN) and bone sialoprotein (BSP),and the... |
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| Keywords: | Actinobacillus actinomycetemcomitans osteoblasts differentiation gap junctional intercellular communication |
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