桂北地区HIV-1感染者HIV-1病毒亚型分布观察

目的观察桂北地区HIV感染者HIV-1病毒亚型分布。方法采集桂北地区80例HIV-1感染者的静脉血,提取其中单个核细胞的DNA,用巢氏PCR扩增病毒膜蛋白env基因的C2-V3区,对PCR纯化产物进行测序,并应用GCG软件等对序列进行分析。结果通过PCR扩增得到env基因序列55份,分属4个亚型,分别为B型1份,CRF07-BC型19份,CRF08-BC型23份,CRF01-AE型8份,C型4份。感染途径为静脉吸毒者16例,性34例,非法献血2例,不详3例。16例吸毒感染者中CRF08-BC型12例,CRF07-BC型4例。34例性行为感染者中CRF07-BC型19例,CRF08-BC型11例,CRF01-AE型4例。结论桂北地区HIV感染者存在HIV-1亚型多样。静脉吸毒感染者以CRF08-BC型为主。性行为感染者以CRF07-BC型和CRF08-BC型为主。

HIV-1 subtype analysis in HIV infections in northern Guangxi

Objective To investigate HIV-1 subtype distribution in in HIV infections in northern Guangxi. Methods Anticoagulant whole blood samples of 80 persons in northern regions of Guangxi, who had been identified to be infected with HIV-1, were collected. The previral DNA was extracted, of which the C2-V3 region of the virus membrane protein ENV gene was amplified by nested polymerase chain reaction （nested-PCR）, then the purified PCR products was equenced. Besides, the sequence was analyzed by GCG software. Results 55 env gene sequences obtained by PCR am- plification were compared with various subtypes of international reference strains of HIV-1 based on ENV gene sequences and were performed phylogenetic analysis, 4 subtypes of HIV of the samples were confirmed, including 1 copy of HIV-1 B subtype, 19 copies of popular restructuring type CRF07-BC, 23 copies of popular recombinant CRF08-BC, 8 copies of epidemic recombinant CRF01-AE and 4 copies of C subtype. Conclusions HIV-1 subtypes are diversitied in northern regions of Guangxi.