健康体检者血小板参数、P-选择素、PAC-1测定及与年龄的相关性分析

目的 探讨增龄对血小板参数及其活化指标的影响和意义.方法 选择同期行健康体检者400例,其中年龄25～44岁156例（成年组）、45～59岁144例（老年前组）、〉60岁（老年组）100例,均用全自动细胞分析仪测定血小板参数包括血小板计数（PLT）、平均血小板体积（MPV）、血小板体积分布宽度（PDW）和血小板压积（PCT）水平,三组各随机选择30例采用流式细胞仪检测P-选择素（CD62P）及血小板膜糖蛋白Ⅱb/Ⅲa（PAC-1）水平,对年龄与上述指标间相关性行Pearson相关分析.结果 随年龄增加PLT、PCT、PDW下降,MPV增大,CD62P、PAC-1增多;PLT与年龄呈明显负相关,MPV、PAC-1、CD62P与年龄呈明显正相关.结论 血小板参数及其活化指标均与年龄有明显相关性,此为血栓性疾病的预防及诊治提供了理论依据.

Measurment and correlation analysis of platelet parameter,P-selection,PAC-1 and age in health examination People

Objective To investigate the effects of aging on the platelet parameter and its active indexes. Methods Four hundreds health examination people were elected ,in which 25 - 44 years 156 cases （adult group）, 45 - 59 years 144 eases （presenium group） and 〉 60 years 100 eases （older age group）, the platelet parameters including platalet（ PLT）, mean platelet volume （MPV）, platelet distribution width （PDW）and plateletcrit （PCT） were measured by automatic blood analyzer, the P-selection（ CD,,P ）and platelet membrane glyeoprotein（ PAC-1 ） were measured by flow cytometry in 30 cases of each group, the correlation between age and aboving indexes were analyzed with Pearson correlation analysis. Rt~LtlIs With the increase of age ,PLT,PCT,PDW decreased, MPV increased, CD62P and PAC-1 increased; PLT had a obviously negative correlation with aging; MPV, PAC-1 and CD62P had a obviously positive correlation with aging. Conclusions Platelct parameters and its active indexes have obviously correlation with age,this provides theory evidence for the prevention, diagnosis and treatment of thromboembolic disease.