HPLC测定板蓝根及其制剂中水杨酸、苯甲酸的含量

目的:建立高效液相色谱法测定板蓝根药材及其制剂中水杨酸、苯甲酸含量方法。方法:以苯甲酸、水杨酸为对照品,采用Lichrospher C18色谱柱(4.6 mm×250 mm,5.0μm),流动相甲醇-0.1%的磷酸溶液(42∶58),流速1 mL.min-1,检测波长230 nm,测定板蓝根药材及其制剂中水杨酸、苯甲酸含量。结果:水杨酸在4.36～21.8 mg.L-1与峰面积线性关系良好(r=0.999 9),苯甲酸在4.04～20.2 mg.L-1内与峰面积线性关系良好(r=0.999 7);水杨酸平均加样回收率为98.45%,RSD1.26%(n=9),苯甲酸平均加样回收率为97.90%,RSD 1.36%(n=9)。结论:该方法简便、准确、重复性好,可用于板蓝根药材及其制剂中苯甲酸、水杨酸的含量测定。

Determination of Salicylic Acid and Benzoic Acid in Radix Isatidis and its Preparations by HPLC

Objective: To determine the content of salicylic acid and benzoic acid in Radix Isatidis and its preparations by HPLC. Method: The content of salicylic acid and benzoic acid in Radix Isatidis and its preparations was determined by HPLC based on a Lichrospher C₁₈ column(4.6 mm×250 mm,5 um)with a gradient elution solvent system composed of methanol -0.1%H₃PO₄ water(42: 58)solution.The flow rate was 1.0 mL·min^-1,and the detection wavelength was set at 230 nm. Result: Salicylic acid had good linearity in the range of 4.36-21.8 mg·L^-1, and the average recovery rate was 98.45% with RSD of 1.26% (n=9),while benzoic acid had good linearity in the range of 4.04-20.2 mg·L^-1,and the average recovery rate was 97.90% with RSD of 1.36% (n=9). Conclusion: The method is simple,stable and reliable,which can be used to determine salicylic acid and benzoic acid in Radix Isatids and its preparations.