| pH、无机盐和有机溶剂对美洲大蠊醇提取物中蛋白质稳定性的影响 |
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| 引用本文： | 吴少辉,罗廷顺,张成桂,巫秀美,刘光明. pH、无机盐和有机溶剂对美洲大蠊醇提取物中蛋白质稳定性的影响[J]. 中国实验方剂学杂志, 2012, 18(21): 74-77 |
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| 作者姓名： | 吴少辉 罗廷顺 张成桂 巫秀美 刘光明 |
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| 作者单位： | 大理学院药学院,云南大理,671000 |
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| 基金项目： | 国家自然科学基金项目(30560181);云南省重点产业创新工程基金项目(2008IF012) |
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| 摘 要： | 目的:对美洲大蠊醇提取物中蛋白质稳定性进行研究.方法:通过采用UV法和Folin-酚试剂法,考察了pH、无机盐和有机溶剂等影响因素对蛋白质稳定性的影响.结果:pH、氯化钠、硫酸铵、乙腈和甲醇随着剂量的增加,在不同程度上破坏了蛋白质的结构.结论:不同的影响因素通过改变蛋白质物理化学性质,破坏了蛋白质的稳定性.
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| 关 键 词： | Folin-酚试剂法 美洲大蠊 紫外分光光度法 |
| 收稿时间： | 2012-05-29 |
Stability of Protein of Ethanol Extract from Periplaneta americana with pH, Inorganic Salt and Organic Solvent |
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| WU Shao-hui,LUO Ting-shun,ZHANG Cheng-gui,WU Xiu-mei and LIU Guang-ming. Stability of Protein of Ethanol Extract from Periplaneta americana with pH, Inorganic Salt and Organic Solvent[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(21): 74-77 |
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| Authors: | WU Shao-hui LUO Ting-shun ZHANG Cheng-gui WU Xiu-mei LIU Guang-ming |
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| Affiliation: | College of Pharmacy, Dali University, Dali 671000, China;College of Pharmacy, Dali University, Dali 671000, China;College of Pharmacy, Dali University, Dali 671000, China;College of Pharmacy, Dali University, Dali 671000, China;College of Pharmacy, Dali University, Dali 671000, China |
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| Abstract: | Objective: Studying the stability of the protein of atitumor activity from Periplaneta americana. Method: The factors of pH, inorganic salt and organic solvent on stability of the protein were studied by the UV and Lowrry method. Result: pH, sodium chloride, ammonium sulfate, acetonitrile and methanol are added and the structure of protein is changed. Conclusion: The physical and chemical properties are changed by different kinds of factors, and finally the stability of the protein is damaged. |
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| Keywords: | lowrry method Periplaneta americana UV method |
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