p27~(kip1)和p57~(kip2)在宫颈癌组织中的表达及其预后意义

目的:探讨细胞周期调控抑制因子p27~(kip1)和p57~(kip2)在宫颈癌发生发展中的作用及预后意义。方法:采用免疫组化二步法检测p27~(kip1)和p57~(kip2)在48例宫颈癌(宫颈癌组)、13例宫颈上皮内瘤变(CIN组)和15例正常宫颈组织(正常组)中的表达。结果:p27~(kip1)和p57~(kip2)在宫颈癌组中的阳性表达率分别为25·00%和20·83%,显著低于正常组和CIN组(P<0·01,P<0·01),而后两组比较差异无统计学意义(P>0·05)。p27~(kip1)表达与宫颈癌病理分化程度显著相关(P<0·01);p57~(kip2)表达与宫颈癌病理分化程度及肿瘤大小有关(P<0·05,P<0·05);两者均与临床分期、组织学类型及淋巴结转移无关(均P>0·05)。p27~(kip1)与p57~(kip2)表达呈极显著正相关(r=0·621,P<0·01)。Kaplan-Meier单因素分析宫颈癌患者生存时间与p27~(kip1)及p57~(kip2)阳性表达有关(P<0·05,P<0·01)。结论:p27~(kip1)和p57kip2的低表达或缺失可能在宫颈癌发生发展中起重要作用,可能与预后不良有关。

Expressions of p27kip1 and p57kip2 in human cervix carcinoma and their prognostic significance

Objective: To investigate the roles of the inhibitory factors of cell cycle regulation p27~ kip1 and p57~ kip2 in the development and advancement of the human cervix carcinoma and their clinicopathological and prognostic significance.Methods :The expressions of p27~ kip1 and p57~ kip2 in tumor tissues of 48 patients with cervix carcinoma and 13cases with cervical intraepithelial neoplasia(CIN) and 15 cases with normal cervical epithelium were detected by Poly-HRP immunohistochemical technique.Results:p27~ kip1 and p57~ kip2 positive-expression rate in tumor tissues of cervix carcinoma was 25.00% and 20.83% respectively,which were lower than those in CIN and normal cervical epithelial tissues(P<0.01,P<0.01),and there was no significant difference in p27~ kip1 and p57~ kip2 staining between normal cervical epithelium and CIN groups(P>0.05).p27~ kip1 positive-expression correlated significantly with tumor cell differentiation(P<0.01).p57~ kip2 positive-expression correlated significantly with tumor cell differentiation and lesion size (P<0.05,P<0.05).p27~ kip1 positive-expression correlated significantly with p57~ kip2 positive-expression (r=0.621,P<0.01).Kaplan-Meier estimation indicated that survival might be related to p27~ kip1 and p57~ kip2 (P<0.05,P<0.01).Conclusion:The decreased expression or loss of p27~ kip1 and p57~ kip2 may play an important role in the development and progression of the cervical carcinoma,which is associated with poor prognosis.