| 弓形虫新基因wx2黄色荧光蛋白标记质粒的构建与鉴定 |
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| 引用本文： | 吴翔,谭逵,张琼,谢荣华,官剑武,舒衡平.弓形虫新基因wx2黄色荧光蛋白标记质粒的构建与鉴定[J].中国现代医学杂志,2008,18(24):3562-3565. |
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| 作者姓名： | 吴翔 谭逵 张琼 谢荣华 官剑武 舒衡平 |
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| 作者单位： | 中南大学湘雅医学院,寄生虫学教研室,湖南,长沙,410078 |
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| 基金项目： | 国家自然科学基金资助项目(No:30371260); 湖南省“十一.五”重大专项(No:2006SK1001); 湖南省“十一.五”重点学科建设经费联合资助(No:03-985-3-7) |
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| 摘 要： | 目的 构建弓形虫新基因wx2黄色荧光蛋白标记的黄色荧光超表达质粒,为进一步研究弓形虫新基因WX2的功能提供工具.方法 采用PCR扩增出编码wx2目的 基因,用BglⅡ,AvrⅡ分别对PCR扩增产物和ptubYFP-YFP/sagCAT进行双酶切.将wx2定向克隆到tubYFP-YFP/sag-CAT;对重组质粒进行PCR,双酶切初步鉴定后做序列测定.结果 特异扩增出预计wx2目的 基因片段,大小为558 bp;扩增产物成功连接到ptubYFP-YFP/sagCAT中,经PCR,双酶切及序列测定结果表明重组质粒中含有基因wx2读框.结论 成功构建弓形虫新基因wx2黄色荧光超表达质粒wx2一tub-YFP sag/CAT.
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| 关 键 词： | 弓形虫 基因wx2 重组质粒 |
Construction and identification of recombinant plasmid marked with YFP of wx2 gene from Toxoplasma gondii |
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| WU Xiang
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TAN Kui
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ZHANG Qiong
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XIE Rong-hua
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GUAN Jian-wu
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SHU Heng-ping.Construction and identification of recombinant plasmid marked with YFP of wx2 gene from Toxoplasma gondii[J].China Journal of Modern Medicine,2008,18(24):3562-3565. |
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| Authors: | WU Xiang TAN Kui ZHANG Qiong XIE Rong-hua GUAN Jian-wu SHU Heng-ping |
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| Institution: | WU Xiang,TAN Kui,ZHANG Qiong,XIE Rong-hua,GUAN Jian-wu,SHU Heng-ping(Department of Parasitology,Xiangya School of Medicine,Central South University,Changsha,Hunan 410078,P.R.China) |
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| Abstract: | Objective] To construct a overexpressed recombinant plasmid marked with YFP of wx2 gene from Toxoplasma gondii,and provide a tool for the researching of the gene function of new gene wx2.Methods] The wx2 opening reading frame(ORF) was amplified with two specific primers removed stop code.The ORF and plasmid p tubYFP-YFP/sagCAT were digested with BglII and AvrII respectively and the ORF was ligated into the ptubYFP-YFP/sagC-AT at the YFP-1 site.The recombinant vector pwx2-tub-YFP sag/CAT was characterized ... |
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| Keywords: | toxoplasma gondii gene wx2 recombinant plasmid |
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