胃癌组织中Maspin 蛋白与血管内皮生长因子-C的表达及其相关性

 目的 探讨胃癌组织中Maspin蛋白与血管内皮生长因子-C（VEGF-C）的表达及其相关性。方法 收集哈尔滨医科大学附属肿瘤医院2002年～2003年间手术切除的胃癌组织石蜡标本61例,采用免疫组化技术检测胃癌组织中Maspin蛋白与血管内皮生长因子-C（VEGF-C）的表达情况,结合临床病理特征进行分析,并探讨两者蛋白表达水平的相关性。结果 Maspin蛋白的阳性表达率为50.8%（31/61）。淋巴结转移阳性组织中Maspin蛋白的阳性表达率明显低于淋巴结转移阴性的组织（32.6%vs94.4%,P=0.000）。且Maspin蛋白在组织分化较低,TNM分期较晚,周围有肿瘤浸润的病例中表达率显著降低（P分别为0.018,0.011和0.028）。VEGF-C在胃癌组织中的阳性表达率为86.9%（53/61）。淋巴结转移阳性组织中VEGF-C蛋白的阳性表达率明显高于淋巴结转移阴性的组织（95.3%vs66.7%,P=0.006）。Maspin蛋白与VEGF-C在胃癌组织的表达存在负相关（Spearmanr=-0.429,P〈0.05）。结论 Maspin蛋白在胃癌组织中低表达,胃癌的浸润转移能力增强可能与Maspin蛋白表达下调、缺失相关;VEGF-C的高表达与肿瘤淋巴结转移关系密切;Maspin蛋白与VEGF-C在胃癌组织中的表达呈明显负相关。

Association between Maspin Protein and Vascular Endothelial Growth Factor-C Expression in Gastric Cancer

Objective 　To explore the association between Maspin protein and Vascular Endothelial Growth Factor-C expression in Gast ric Cancer. Methods 　Maspin and VEGF-C proteins were detected in formalin-fixed paraffin-embedded tissue specimens from 61 cases of gastric cancer tissues treated from 2002 to 2003 in Tumor Hospital affiliated to Harbin Medical Univercity by S-P immunohistochemical method. To analyze the level of their expression different clinical and pathological characteristics . Results 　The positive rate of maspin protein expression was 50. 8 %(31/ 61) in gasrci carcinoma 。Maspin expression in gastric carcinoma with lymph node metastasis was significantly lower than that in those without metastasis (32. 6 % vs 94. 4 % , P = 0. 000) , and down regulation of the expression level was associated with poorly differentiated phenotype , late TNM stage and direct invasion around stamoch ( P = 0. 018 , 0. 011 and 0. 028 , respectively) . The positive rate of VEGF-C expression was 86. 9 %(53/ 61) in gast ric carcinoma . The positive rate of VEGF-C expression in gast ric cancerous tissues with lymph node metastasis was higher than that in non-metastasis (95. 3 % vs 66. 7 % , P = 0. 006) . The expression of maspin protein was negative associated with the expression of VEGF-C by statistically analysis ( spearman r = 0. 429 P =0. 001) . Conclusion 　Expression of maspin shows down regulation in gast ric cancer . It is correlated to the malignant biological behaviors of gastric carcinoma. VEGF-C is well associated with proliferation and dilation of lymphatic vessels. There is a negative correlation between the expression of Maspin and VEGF-C.