木蝴蝶总黄酮的大孔吸附树脂纯化工艺

目的:优选木蝴蝶总黄酮的大孔吸附树脂分离纯化工艺,并确定最佳工艺参数.方法:采用静态与动态吸附-洗脱相结合的方法,以吸附率与洗脱率为指标,考察了5种树脂对木蝴蝶总黄酮的纯化能力.结果:HPD100型树脂的吸附率与解吸率均较高.动态吸附-洗脱的最佳条件:上样液质量浓度4.618 g?L-1,吸附流速3.0 mL? min -1,溶液pH 2.0,3 BV水洗,解析剂为5 BV 90％乙醇.结论:在最佳工艺条件下,HPD100型大孔吸附树脂纯化效果良好,所得木蝴蝶纯化物中总黄酮质量分数＞80％.

Purification Technology of Total Flavones from Oroxylum indicum by Macroporous Resin

Objective: To optimize separation and purification technology of total flavones form Oroxylum indicum by macroporous resin,and establish optimum technology parameters. Method: Purification capacity of 5 different macroporous resins for total flavones form O. indicum with absorption ratio and desorption ratio as indexes by combining static and dynamic adsorption-desorption method. Result: HPD100 resin possessed good adsorption capacity and desorption rate by comparing with other resins.Optimized conditions of dynamic adsorption-desorption experiment were:concentration of sample was 4.618 g·L^-1 (pH 2.0) at absorption flow speed of 3.0 mL·min^-1,washed with 3 BV water and eluted with 5 BV of 90% ethanol. Conclusion: Under this optimized technology,HPD100 macroporous resin could be used to purify total flavones of O. indicum,the content of total flavones was more than 80%.