脑脊液流式细胞学检测在急性淋巴细胞白血病并发中枢神经系统白血病中的诊断价值

目的:探讨分析脑脊液流式细胞学(FCM)检测在急性淋巴细胞白血病(ALL)并发中枢神经系统白血病（CNSL）中的诊断价值。方法:选择2012年1月至2016年6月我院收治的89例ALL患者脑脊液标本，采用脑脊液FCM技术、脑脊液细胞学（CC）法进行检测，对比分析两种检测方式对CNSL的诊断情况。结果:经脑脊液FCM检出23例异常表型细胞，检出阳性率为25.84%，经脑脊液CC检出10例异常细胞，检出阳性率为11.24%，脑脊液FCM检出阳性率明显高于脑脊液CC检查（P＜0.05）。对于脑压增高患者，FCM检出阳性率明显高于CC检出阳性率（P＜0.05）；对于脑脊液糖正常患者，FCM检出阳性率明显高于CC检出阳性率（P＜0.05）。结论:脑脊液FCM在ALL并发CNSL诊断中具有着重要的临床意义，是脑脊液CC检查的重要补充方法。

The diagnosis value of cerebrospinal fluid flow cytology in acute lymphoblastic leukemia complicated with central nervous system leukemia

Objective:To explore the value of cerebrospinal fluid(CSF)flow cytologic diagnosis acute lymphoblastic leukemia complicated with central nervous system disease.Methods:The CSF samples from 89 cases with ALL diagnosed in our hospital from January 2012 to June 2016,and cerebrospinal fluid flow cytometry(FCM)and cerebrospinal fluid cytology(CC)were used to detect the diagnosis of CNSL.The diagnosis of CNSL by two methods was compared.Results:23 cases of abnormal phenotypic cells were detected by cerebrospinal fluid FCM.The positive rate was 25.84%.10 cases of abnormal cells were detected by CSF cell morphology,and the positive rate was 11.24%.The positive rate of CSF FCM detection was significantly higher than that of CSF cell morphology(P<0.05).For patients with increased brain pressure,the positive rate of FCM detection was significantly higher than that of cell morphology detection(P<0.05).For patients with normal CSF,the positive rate of FCM detection was significantly higher than that of cell morphology detection(P<0.05).Conclusion:The FCM of cerebrospinal fluid is of important clinical significance in the diagnosis of CNSL with ALL,and is an important supplementary method for the morphological examination of cerebrospinal fluid cells.