| 膜联蛋白A7在胃癌组织中的表达和意义 |
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| 引用本文： | 张扬许倩 陈龙 王小杰 田焕娜 李春辉李欣. 膜联蛋白A7在胃癌组织中的表达和意义[J]. 解剖学报, 2015, 46(4): 528-531. DOI: 10.16098/j.issn.0529-1356.2015.04.014 |
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| 作者姓名： | 张扬许倩 陈龙 王小杰 田焕娜 李春辉李欣 |
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| 作者单位： | 1.承德医学院基础医学研究所,河北 承德 067000; 2.湖南省衡阳市中心医院病理科，湖南 衡阳 421001; 3.承德医学院附属医院病理科， 河北 承德 067000 |
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| 摘 要： | 目的 检测膜联蛋白A7（ANXA7）在胃癌组织中的表达改变，探讨胃癌的发生机制。 方法 收集人胃癌组织55例及正常胃组织25例，细胞裂解液裂解组织细胞，蛋白定量试剂盒进行蛋白定量后，Western blotting法和Real-time PCR法分别于蛋白水平和基因转录水平检测ANXA7在胃癌组织和正常胃组织中的表达改变，并用免疫组织化学法对ANXA7在细胞中的定位和表达进行检测。 结果 ANXA7在胃癌中蛋白水平和转录水平的表达均显著增高，与正常组相比差异有显著性（P<0.05）； ANXA7在胃癌细胞中定位于细胞质。 结论 膜联蛋白A7在胃癌中的表达增高有可能是胃癌发生的机制之一。
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| 关 键 词： | 膜联蛋白A7 胃癌 免疫印迹法 实时定量聚合酶链反应 人 |
| 收稿时间： | 2014-10-20 |
| 修稿时间： | 2015-01-19 |
Expression of annexin A7 in human gastric carcinomas and the clinic significance |
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| HANG Yang XU Qian CHEN Long WANG Xiao-jie TIAN Huan-na LI Chun-hui LI Xin. Expression of annexin A7 in human gastric carcinomas and the clinic significance[J]. Acta Anatomica Sinica, 2015, 46(4): 528-531. DOI: 10.16098/j.issn.0529-1356.2015.04.014 |
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| Authors: | HANG Yang XU Qian CHEN Long WANG Xiao-jie TIAN Huan-na LI Chun-hui LI Xin |
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| Affiliation: | 1. Institute of Basic Medical Sciences, Chengde Medical College, Hebei Chengde 067000, China; 2. Department of Pathology, Central Hospital in Hengyang Hunan, Hu’nan Hengyang 421001, China; 3. Department of Pathology, Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China |
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| Abstract: | Objective To dectect the expression of annexin A7 in human gastric carcinoma so as to detect the mechanism. Methods Fifty five gastric carcinoma samples and 25 normal human gastric samples were collected. Each sample was lysed in lysis buffer. Whole protein of the supernatant was estimated by BCA-100 Protein Quantitative Analysis Kit. The expression of annexin A7 in human gastric carcinoma and normal gastric tissue was examined with Western blotting and Real|time PCR. Immunehistolchemistry was used to detect the expression position of annexin A7 in the gastric carcinoma cells. Results The expression of annexin A7 increased in gastric cancer not only on protein level but also on RNA level when compared with normal gastric tissues, the difference was significant (P<0.05); Annexin A7 mainly expressed in the cytoplasm of the gastric carcinoma cells. Conclusion Expression of annexin A7 is up-regulated in human gastric cancer, and it may involve in the development of gastric cancer. |
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| Keywords: | Annexin A7 Gastric cancer Western blotting Real-time PCR Human |
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