| 结核分枝杆菌km基因的突变情况分析 |
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| 引用本文： | 王金河,孙海林,李洪敏,梁建琴,冯士生.结核分枝杆菌km基因的突变情况分析[J].中国实验诊断学,2010,14(10):1542-1544. |
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| 作者姓名： | 王金河 孙海林 李洪敏 梁建琴 冯士生 |
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| 作者单位： | 1. 解放军309医院结研所,结核二科,北京,100091
2. 鄂尔多斯二院,结核科 |
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| 摘 要： | 目的探讨结核分枝杆菌km基因突变的情况。方法通过传统梯度药敏试验和聚合酶链反应-单链构象多态性(PCR-SSCP)分析248株分枝杆菌临床分离株耐药情况和km基因的变化。结果药敏试验耐药率为32.7%(81/248),248株分枝杆菌临床分离株的16S rDNA SSCP电泳图谱均与结核分枝杆菌标准株相同。81株耐卡那霉素分离株中,24株(29.6%)km基因SSCP泳动异常。结论其部分耐药原因可能是由于T.M耐KM药物的km基因突变所致。
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| 关 键 词： | 结核分枝杆菌 卡那霉素 km基因 |
The research of the relatonship between Km gene mutation and the drug-resistance of M.tuberculosis |
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| Institution: | WANG Jin-he,SUN Hai-lin,LI Hong-min,et al.(Tuberculosis Research Institute,309th Hospital of PLA,Beijing 100091,China) |
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| Abstract: | Objective To study the relationship between Km gene mutation and the drug-resistant M.tuberculosis.Methods Kanamycin drug-resistance and Km gene mutation in 248 M.tuberculosis strains isolated from clinical sputum specimens were analyzed by traditional drug susceptibility tests and PCR-SSCP.Results The rate of drug susceptibility tests was 32.7%（81/248）.The electrophoresis profiles of 16S rDNA PCR-SSCP indicated that the 248 M.tuberculosis strains were according to M.TB standard strain.24 of the 81 kanamycin drug-resistant strains（29.6%） displayed abnormal in PCR-SSCP profiles.Conclusion In some degree,Km gene mutation made resistance to kanamycin of M.tuberculosis strains. |
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| Keywords: | M tuberculosis kanamycin Km gene |
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