增殖和凋亡基因在喉上皮良恶性病变中的检测及其意义

目的 :探讨增殖相关基因Ki 6 7和凋亡相关基因Bax、Bcl 2在喉上皮良、恶性疾病中的表达和临床意义。方法 :采用免疫组织化学SP法检测存档石蜡标本喉癌、不典型增生、喉乳头状瘤、声带息肉、喉正常粘膜组织中Ki 6 7和Bax、Bcl 2表达。结果 :Ki 6 7在以上组织中的表达率依次为 5 2 .0 %、5 0 .0 %、4 7.8%、4 1.7%、10 .0 % ,喉癌明显高于喉正常粘膜 4 4 .0 % (P <0 .0 5 ) ,不典型增生明显高于喉正常粘膜 (P <0 .0 5 )。Bcl 2的表达率在不典型增生为 11.0 % ,在喉癌中为 4 4 .0 % ,其余 3组为 0 %。喉癌表达明显高于不典型增生和正常粘膜(P <0 .0 5 ) ,极显著高于喉乳头状瘤和声带息肉 (均P <0 .0 1)。Bax在各组织中表达率依次为 76 .0 %、77.8%、6 9.6 %、5 8.3%、80 .0 % ,各组差异无显著性意义。在喉乳头状瘤中Ki 6 7、Bcl 2表达成正相关 (P <0 .0 5 )。结论 :Ki 6 7、Bcl 2在喉乳头状瘤中的协同表达提示细胞内无法控制的增殖 ;二者有可能是癌变的前提 ,在癌前病变中有一定的诊断价值

Expression and significance of proliferation marker and apoptosis-related genes in benign and malignant epithelial lesions of the larynx

OBJECTIVE: In order to study the ki-67, bcl-2, bax proteins expression and significance in the benign and malignant epithelium of the larynx. METHOD: An immunohistochemical S-P assay was used to detect the expression of ki-67, bcl-2, bax in LSCCs, epithelial dysplasia, papillomas, simple hyperplasia and normal laryngeal epithelium. RESULT: The mean value of ki-67 was 52.0%, 50.0%, 47.8%, 41.6%, 10.0% respectively. The different was statistically significant between LSCCS or dysplasia and normal laryngeal epithelial (P < 0.05). The mean value of bcl-2 was 44.0% in LSCCs, 11.0% in dysplasia, 0% in other three epithelial lesions of the larynx. Bcl-2 was highly positive in LSCCs compared to the other lesions (P < 0.05, P < 0.01, P < 0.01). The expression of bax was 76.0%, 77.8%, 69.6%, 58.3%, 80.0%. In papillomas, there was statistically significant positive correlation between Bcl-2 and ki-67. CONCLUSION: Ki-67 and Bcl-2 coexpression may play a role in the uncontrolled proliferation in papillomas. Ki-67, bax, Bcl-2 could help in estimation the premalignant stages of the larynx.