| HBV—DNA分子杂交技术的建立及其临床方面的应用 |
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| 引用本文： | 寇平原,买凯,洪昌睦,朱金凤,王滨,申庆红,金志宏,陈京. HBV—DNA分子杂交技术的建立及其临床方面的应用[J]. 郑州大学学报(医学版), 1986, 0(2) |
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| 作者姓名： | 寇平原 买凯 洪昌睦 朱金凤 王滨 申庆红 金志宏 陈京 |
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| 作者单位： | 河南医学科学研究所肝炎研究室(寇平原，买凯，洪昌睦，朱金凤，王滨，申庆红，金志宏)，河南医学科学研究所肝炎研究室(陈京) |
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| 摘 要： | 本文采用目前最灵敏的HBV—DNA分子杂交技术,利用α—~(32)P和生物素标记DNA做为探针进行乙型肝炎的研究。对230例HBsAg阳性的携带者进行检测HBV—DNA阳性者占26.5%。230例中HBeAg占63%,抗—HBe占25.2%。而HBV—DNA阳性的血清中HBeAg阳性占86.9%为阳性。抗—HBe中只有8.1%。两者有显著差异。本文还对HBV—DNA与HBsAg免疫复合物的关系以及乙肝疫苗的检测有一些新的评价。
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| 关 键 词： | 乙型肝炎病毒DNA 乙型肝炎表面抗原 乙型肝炎e抗原 |
The technique of HBV-DNA molecular hybridization and its application in clinical medicine |
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| Kou Ping yuan,et al Henan Institute of Medical Sciences. The technique of HBV-DNA molecular hybridization and its application in clinical medicine[J]. Journal of Zhengzhou University: Med Sci, 1986, 0(2) |
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| Authors: | Kou Ping yuan et al Henan Institute of Medical Sciences |
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| Abstract: | The most sensitive technique of molecular hybridization and the probes labelled with alpha-~(32)P and biotin to detect HBV-DNA were used in the study of hepatitis B. Two hundred and thirty HBsAg carriers were examined and 26.5 percent of them were positive in HBV-DNA. Of the 230 cases, 63 percent were HBeAg-positive and 25.2 pereet Anti-HBe-positive and 8.1 percent Anti-HBe-positive.The difference between them is significant. Some new results were derived from using the technique to detect HBV-DNA, HBsAg immune complex, and hepatitis B vaccine at the same time. |
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| Keywords: | Hepatitis B virus DNA (HBV-DNA) HBsAg HBeAg |
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