| TRAIL对人肺腺癌细胞株Calu-3增殖和凋亡的影响 |
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| 引用本文： | 王 晗,吴异兰,解方为. TRAIL对人肺腺癌细胞株Calu-3增殖和凋亡的影响[J]. 肿瘤学杂志, 2014, 20(5): 369-372 |
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| 作者姓名： | 王 晗 吴异兰 解方为 |
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| 作者单位： | 南京军区福州总医院;福建中医药大学;南京军区福州总医院 |
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| 基金项目： | 国家自然科学基金（81274002）；福建省自然科学基金（2012J01383）；福建省自然科学基金面上项目（2010J01222） |
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| 摘 要： | [目的]研究肿瘤坏死因子相关凋亡诱导配体（tumor necrosis factor-related apoptosis-inducing ligand,TRAIL）对人肺腺癌细胞株Calu-3增殖和凋亡的影响。[方法]用不同浓度TRAIL分不同时间段干预Calu-3细胞,MTT法检测细胞增殖抑制率,流式细胞仪检测细胞凋亡率。[结果]Calu-3细胞生长被TRAIL抑制,具有浓度和时间依赖性。TRAIL能诱导Calu-3细胞凋亡。[结论]TRAIL在体外具有抑制Calu-3细胞增殖,促使Calu-3细胞凋亡的作用。
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| 关 键 词： | 肺肿瘤 肿瘤坏死因子相关凋亡诱导配体 细胞增殖 细胞凋亡 |
| 收稿时间： | 2014-01-12 |
Effect of TRAIL on the Proliferation and Apoptosis of Human Lung Adenocarcinoma Cell Line Calu-3 |
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| Affiliation: | Fuzhou General Hospital of Nanjing Military Region |
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| Abstract: | [ Purpose ] To investigate the effect of TNF-related apoptosis inducing ligand （TRAIL） on the proliferation and apoptosis of the human lung adenocarcinoma cell line Calu-3. [Methods ] Calu ceils were treated with different concentrations of TRAIL at different times. Inhibition of proliferation and apoptosis of Calu-3 cells were detected by MTY assay and flow cytometry. [Results] The proliferation of Calu-3 cells were inhibited by TRAIL in a dose-time dependent manner. Apoptosis of Calu-3 cells was induced by TRAIL. [Conclusion] TRAIL could inhibit Calu-3 cells proliferation and induce cell apoptosis in vitro. |
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| Keywords: | lung neoplasms TRAIL cell proliferation cell apoptosis |
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