| 异体恒河猴视网膜色素上皮细胞移植探讨 |
| |
| 引用本文： | 徐国兴,郭健,丁静文,周建华.异体恒河猴视网膜色素上皮细胞移植探讨[J].医学研究杂志,2008,37(9). |
| |
| 作者姓名： | 徐国兴 郭健 丁静文 周建华 |
| |
| 作者单位： | 1. 福建医科大学附属第一医院/福建省眼科研究所,福州,350005
2. 福建省计划生育科学技术研究所非人灵长类动物实验中心,福州,350005 |
| |
| 基金项目： | 国家人事部留学回国人员优秀课题基金，福建省重大科研课题基金 |
| |
| 摘 要： | 目的 观察供体视网膜色素上皮细胞(retinal pigment epithelium,RPE)在视网膜下腔生长情况,为视网膜色素上皮细胞移植的临床应用提供实验基础.方法 体外获取恒河猴视网膜色素上皮细胞经传代培养至第3代,用5-溴脱氧尿嘧啶标记后经外路将供体色素上皮细胞移植到受体恒河猴视网膜下腔,用光镜和电镜观察移植细胞的存活情况.结果 供体细胞在受体视网膜下腔形成有极性的单细胞层,贴附于Brueh膜上,并形成微绒毛和基底内褶,细胞内可见吞噬体.结论 外路法是一种切实可行的视网膜色素上皮移植方法,移植后的细胞可存活并恢复正常的结构和功能.
|
| 关 键 词： | 恒河猴 视网膜色素上皮细胞 移植 外路法 |
The Study of Rhesus Retinal Pigment Epithelium Cells Transplantation |
| |
| Xu Guoxing,Guo Jian,Ding Jingwen,Zhou Jianhua.The Study of Rhesus Retinal Pigment Epithelium Cells Transplantation[J].Journal of Medical Research,2008,37(9). |
| |
| Authors: | Xu Guoxing Guo Jian Ding Jingwen Zhou Jianhua |
| |
| Institution: | Xu Guoxing,Guo Jian,Ding Jingwen,Zhou Jianhua.Department of Ophthalmology,The First Affiliated Hospital of Fujian Medical University,Fujian 350005,China |
| |
| Abstract: | Objective To investigate the retinal pigment epithelium cells(RPE) and makeit the base of clinical application for transplantation.Methods The cultured RPE cells labeled by BrdU was injected into the host's subretinal space by external transcleral.The structure and ultrastructure of the transplanted RPE cells were observed by microscope and transmission electron microscope.Results The transplanted cells had the normal structure as the host's cells.They attached to Bruch's membrane with basal infoldings and ... |
| |
| Keywords: | Rhesus Retinal pigment epithelium(RPE) Transplantation External transcleral |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《医学研究杂志》浏览原始摘要信息 |
| 点击此处可从《医学研究杂志》下载免费的PDF全文 |
|
