小红参对心肌缺血/再灌注损伤NO、氧化应激及线粒体能量代谢的影响*

目的研究小红参对心肌缺血/再灌注损伤中NO、氧化应激及线粒体能量代谢的影响。方法 选取Wistar雄性大鼠48只随机分为假手术组、模型组、复方丹参片组（300 mg/kg）、小红参低、中、高剂量组（56.7、170、280 mg/kg）。各组预防性给药14 d,除假手术组外,其余各组均通过结扎心脏冠状动脉左前降支30 min,再灌注2 h的方法,复制心肌缺血/再灌注损伤模型。分别使用试剂盒检测血清中NO含量、eNOS活性和心肌组织中ROS、CAT、T-AOC、ATP的含量及心肌细胞线粒体ATP酶和膜电位的变化。结果 与假手术组比较,模型组中NO、CAT、T-AOC的含量和eNOS的活性,线粒体能量代谢ATP酶及心肌细胞的线粒体膜电位均下降,心肌组织中ROS水平升高（P<0.05）。与模型组比较,小红参低剂量组对Na+-K+-ATP酶和心肌细胞线粒体膜电位变化差异无统计学意义,而复方丹参片组和其它小红参剂量组均能提高NO、CAT、T-AOC的含量和eNOS的活性及线粒体能量代谢ATP酶和心肌细胞线粒体的膜电位,同时降低心肌组织中ROS水平（P<0.05）。结论 小红参对心肌缺血/再灌注损伤具有保护作用,其机制可能与升高血清中NO水平、抗氧化及改善线粒体能量代谢有关。

The Effect of Rubia Yunnanensis on NO, Oxidative Stress and Mitochondrial Energy Metabolism in Myocardial Ischemia/Reperfusion Injury

Objective To study the effects of Rubia yunnanensis on NO, oxidative stress and mitochondrial energy metabolism in myocardial ischemia/reperfusion injury. Methods 48 male Wistar rats were randomly divided into sham operation group, model group, Fufang Danshen Tablet group, low, middle and high doses of Rubia yunnanensis groups. After administrated for 14 d, all rats except the sham group were made myocardial ischemia/reperfusion injury model by ligating the left anterior descending branch of the coronary artery for 30 min and reperfusion for 2 h. The content of NO and the activity of eNOS in serum, and the levels of ROS, CAT, T-AOC and ATP in myocardial tissue, and the changes of mitochondrial ATPase and membrane potential in myocardial cells were detected. Results Compared with the sham group, the contents of NO, CAT, T-AOC, the activity of eNOS, the mitochondrial energy metabolizing ATPase and the mitochondrial membrane potential of cardiomyocytes in the model group decreased, and the level of ROS in myocardial tissue increased (P<0.05). There were no significant difference in Na+-K+-ATPase and mitochondrial membrane potential both in low Rubia yunnanensis dose group and model group, while the content of NO, the activities of CAT, T-AOC, eNOS, mitochondrial energy metabolizing ATPase and mitochondrial membrane potential in Fufang Danshen Tablet group and other Rubia yunnanensis treatment groups were all increased, and the level of ROS were decreased (P<0.05). Conclusion Rubia yunnanensis has protective effect on myocardial ischemia/reperfusion injury, and its mechanism may be related to the increase of serum NO level, antioxidation and improvement of mitochondrial energy metabolism.