Trichomonicidal activity of a new anthraquinone isolated from the roots of Morinda panamensis Seem |
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Authors: | David Cáceres-Castillo Yussel Pérez-Navarro Julio César Torres-Romero Gumersindo Mirón-López Jimmy Ceballos-Cruz Victor Arana-Argáez Laura Vázquez-Carrillo José Manuel Fernández-Sánchez María Elizbeth Alvarez-Sánchez |
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Affiliation: | 1. Laboratorio de Química Farmacéutica. Facultad de Química, Universidad Autónoma de Yucatán, Mérida, Yucatan, Mexico;2. Posgrado de Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Mexico, D.F., Mexico;3. Laboratorio de Bioquímica y Genética Molecular. Facultad de Química, Universidad Autónoma de Yucatán, Mérida, Yucatan, Mexico;4. Laboratorio de Farmacología. Facultad de Química, Universidad Autónoma de Yucatán, Mérida, Yucatan, Mexico;5. Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Ciudad de México, Mexico |
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Abstract: | Trichomoniasis, caused by the protozoan parasite Trichomonas vaginalis, is the most common nonviral sexually transmitted infection worldwide. Although drug treatment is available, unpleasant side effects and increased resistance to the nitroimidazole family have been documented. Hence, there is a need for the identification of new and safe therapeutic agents against T. vaginalis. Antimicrobial activity of anthraquinone compounds has been reported by a number of authors. The genus Morinda is well known for the diversity of anthraquinones with numerous biological activities. A new anthraquinone, lucidin-ω-isopropyl ether, was isolated from the roots of Morinda panamensis Seem. The structure of the compound was determined by 1H and 13C Nuclear Magnetic Resonance (NMR) analyses, in addition to comparison with literature reports. Using in vitro susceptibility assay, the half inhibitory concentration (IC50) of lucidin-ω-isopropyl ether for T. vaginalis (1.32 μg/mL) was found similar to that of metronidazole concentration tested (6 μM = 1.03 μg/mL). In addition, this anthraquinone was capable of inhibiting the parasite's ability to kill HeLa cells and decreased proteolytic activity of the proteinase TvMP50 from T. vaginalis. This was associated with the decreased expression of the mp50 gene. These results demonstrate the trichomonicidal potential by lucidin-ω-isopropyl ether. Further action-mode studies are necessary to elucidate the antiparasitic mechanism of this new anthraquinone to develop a more potent antitrichomonal agent. |
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Keywords: | anthraquinone antiparasitic Morinda panamensis Trichomonas vaginalis |
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