Study of canine parvovirus evolution: comparative analysis of full-length VP2 gene sequences from Argentina and international field strains |
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Authors: | Marina Gallo Calderón Maximiliano Wilda Lorena Boado Leticia Keller Viviana Malirat Marcela Iglesias Nora Mattion Jose La Torre |
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Institution: | (1) Centro de Virolog?a Animal, Instituto de Ciencia y Tecnolog?a Dr. Cesar Milstein CONICET, Saladillo 2468, C1440FFX Buenos Aires, Argentina;(2) Fundaci?n de Estudios en Virolog?a Animal (FEVAN), Guamin? 1682, C1440ESD Ciudad de Buenos Aires, Argentina; |
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Abstract: | The continuous emergence of new strains of canine parvovirus (CPV), poorly protected by current vaccination, is a concern
among breeders, veterinarians, and dog owners around the world. Therefore, the understanding of the genetic variation in emerging
CPV strains is crucial for the design of disease control strategies, including vaccines. In this paper, we obtained the sequences
of the full-length gene encoding for the main capsid protein (VP2) of 11 canine parvovirus type 2 (CPV-2) Argentine representative
field strains, selected from a total of 75 positive samples studied in our laboratory in the last 9 years. A comparative sequence
analysis was performed on 9 CPV-2c, one CPV-2a, and one CPV-2b Argentine strains with respect to international strains reported
in the GenBank database. In agreement with previous reports, a high degree of identity was found among CPV-2c Argentine strains
(99.6–100% and 99.7–100% at nucleotide and amino acid levels, respectively). However, the appearance of a new substitution
in the 440 position (T440A) in four CPV-2c Argentine strains obtained after the year 2009 gives support to the variability
observed for this position located within the VP2, three-fold spike. This is the first report on the genetic characterization
of the full-length VP2 gene of emerging CPV strains in South America and shows that all the Argentine CPV-2c isolates cluster
together with European and North American CPV-2c strains. |
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