|
|||||
|
|
| NDRG2调控乳腺癌细胞侵袭能力的机制研究 | |
| 引用本文： | 郑瑾,杨建栋,史恒军,任秦有,丁井永,刘新平,刘文超.NDRG2调控乳腺癌细胞侵袭能力的机制研究[J].山西医科大学学报,2011,42(8):629-632,694. |
| 作者姓名： | 郑瑾 杨建栋 史恒军 任秦有 丁井永 刘新平 刘文超 |
| 作者单位： | 1. 第四军医大学西京医院肿瘤中心,西安,710032;第四军医大学唐都医院中医科暨中西医结合肿瘤科 2. 第四军医大学西京医院肝胆外科 3. 第四军医大学唐都医院中医科暨中西医结合肿瘤科 4. 第四军医大学生物化学与分子生物学教研室 5. 第四军医大学西京医院肿瘤中心,西安,710032 |
| 基金项目： | 国家自然科学基金资助项目(81072973) |
| 摘 要： | 目的阐明NDRG2(N-Myc downstream-regulated gene 2)在乳腺癌细胞中对CD24的调控及其对乳腺癌细胞侵袭能力的影响。方法 RT-PCR和Western blot检测乳腺癌细胞MCF-7及Bcap-37中NDRG2和CD24的表达;通过腺病毒载体上调MCF-7细胞中NDRG2的水平,或利用siRNA下调Bcap-37细胞中NDRG2的表达,检测CD24的变化以及细胞侵袭能力的改变。结果 MCF-7细胞中NDRG2基因和蛋白的表达水平低于Bcap-37细胞,而CD24的表达水平高于Bcap-37细胞;在MCF-7细胞中上调NDRG2可以抑制CD24的表达并抑制其侵袭能力,而在Bcap-37细胞中下调NDRG2的表达可以提高CD24的水平及细胞的侵袭能力。结论上调NDRG2能够降低CD24的表达,减弱乳腺癌细胞的侵袭能力。 |
| 关 键 词： | NDRG2 CD24 乳腺癌细胞 侵袭 |
Study on NDRG2 inhibiting the invasion potential of breast cancer cells |
|
| ZHENG Jin,YANG Jian-dong,SHI Heng-jun,REN Qin-you,DING Jing-yong,LIU Xin-ping,LIU Wen-chao.Study on NDRG2 inhibiting the invasion potential of breast cancer cells[J].Journal of Shanxi Medical University,2011,42(8):629-632,694. | |
| Authors: | ZHENG Jin YANG Jian-dong SHI Heng-jun REN Qin-you DING Jing-yong LIU Xin-ping LIU Wen-chao |
| Institution: | ZHENG Jin1,2,YANG Jian-dong3,SHI Heng-jun2,REN Qin-you2,DING Jing-yong2,LIU Xin-ping4,LIU Wen-chao1*(1Dept of Oncology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China,2Dept of Integrated Traditional and Western Medicine of Oncology,Tangdu Hospital,3Dept of Hepatobiliary Surgery,4Dept of Biochemistry and Molecular Biology,Fourth Military Medical University) |
| Abstract: | Objective To explore the effect of N-Myc downstream-regulated gene 2(NDRG2) on CD24 expression in breast cancer cells and the invasion potential of cancer cells.Methods NDRG2 and CD24 expressions in MCF-7 and Bcap-37 cells were detected by RT-PCR and Western blot.CD24 expression and cell invasion ability were detected after NDRG2 was up-or down-regulated by adenovirus or siRNA treatment.Results Both the gene and protein expressions of NDRG2 in MCF-7 cells were lower than that in Bcap-37 cells,while CD24 exp... |
| Keywords: | N-Myc downstream-regulated gene 2 CD24 breast carcinoma cell invasion |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |