Novel observation of hot-cold-hot hemolysis exhibited by group B streptococci |
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| Authors: | Okazaki Norio Osawa Ro Suzuki Rieko Nikkawa Takayasu Whiley Robert A |
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| Institution: | Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Nakao 1-1-1, Asahi-ku, Yokohama 241-0815, Japan. |
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| Abstract: | Six human isolates of group B streptococci (GBS) were cultured on blood agar anaerobically at 37 degrees C for 18 h and then at 4 degrees C for 6 h and reincubated anaerobically at 37 degrees C for 6 h. Three of the strains showed a marked enlargement of the hemolysis zone compared with that obtained after hot-only (37 degrees C for 18 h) or hot-cold (37 degrees C for 18 h and then 4 degrees C for 6 h) treatment. Subsequent broth culture experiments revealed that enhanced hemolytic activity due to hot-cold-hot treatment was observed in all 6 GBS strains when cultured in the presence of starch. |
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