| 两性霉素B合并氟康唑对体外培养新生隐球菌酚氧化酶的影响 |
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| 引用本文： | 刘卫兵,温海,岳喜昂,廖万清. 两性霉素B合并氟康唑对体外培养新生隐球菌酚氧化酶的影响[J]. 中国临床药学杂志, 2003, 12(2): 79-81 |
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| 作者姓名： | 刘卫兵 温海 岳喜昂 廖万清 |
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| 作者单位： | 1. 中国人民解放军404医院皮肤科,威海,264200
2. 中国人民解放军第二军医大学附属长征医院皮肤科,上海,200003 |
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| 摘 要： | 目的：研究两性霉素B合并氟康唑对体外培养新生隐球菌酚氧化酶活性的影响。方法：Novozyme234酶消化法获取原生质体；冷冻玻璃珠粉碎原生质体；Polecheck法测定酚氧化酶活性。结果：两性霉素B合并氟康唑组的新生隐球菌酚氧化酶活性较空白对照组明显降低（P＜0．001），但在大于和接近最低抑菌浓度上限的组间无明显差异。结论：两性霉素B合并氟康唑可降低新生隐球菌酚氧化酶活性，在大于或接近最低抑菌浓度上限时与药物浓度无关。
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| 关 键 词： | 两性霉素B 氟康唑 体外培养 新生隐球菌 酚氧化酶 |
| 文章编号： | 1007-4406(2003)02-0079-03 |
Effect of amphotericin B united with fiuconazole on Cryptococcus neoformans phenoloxidases activity |
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| Abstract: | AIM: To study the effect of amphotericin united with fluconazole on Cryptococcus neoformans phenoloxidase activity. METHODS: Protoplast was acquired by Novozyme 234 enzyems digestion. Protoplast was broken by glass beads in freezing condition. Phenoloxidase activity was detected as Polecheck. RESULTS: The phenoloxidase activity of amphotericin B and fluconazole group was lower than control group ( P <0 001). But there wasn't significant difference when amphotericin B and fluconazole concentrations reached or exceeded the MICs. CONCLUSION: Amphotericin B united with fluconazole can decrease the phenoloxidase activity of cryptococccus neoformans . When the concentrations are near or higher than MICs, the activities have nothing to do with durgs concentrations. |
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| Keywords: | amphotericin B fluconazole Cryptococcus neoformans phenoloxidase |
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