hsa-miR-520h downregulates ABCG2 in pancreatic cancer cells to inhibit migration,invasion, and side populations |
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Authors: | F Wang X Xue J Wei Y An J Yao H Cai J Wu C Dai Z Qian Z Xu Y Miao |
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Affiliation: | 1.Laboratory of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009, PR China;2.Center for Pancreatic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009, PR China;3.Department of General Surgery, The First Affiliated Hospital of Yangzhou University, Yangzhou 225001, PR China |
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Abstract: | Background: Expression of ABCG2 is normally absent or low in the pancreas, but high in human pancreatic cancer cells. The mechanism by which ABCG2 is altered in human cancers remains unknown.Methods: We investigated ABCG2 expression in four pancreatic cancer cell lines, and used three microRNA (miRNA) target prediction programmes, and information from the existing literature to predict and identify hsa-miR-520h as an miRNA that targets ABCG2. The function of this miRNA was investigated by transient transfection of the pancreatic cancer cell line PANC-1 with oligonucleotides that mimic hsa-miR-520h.Results: Results showed that both mRNA and protein levels of ABCG2 were reduced, indicating that it was a target of hsa-miR-520h. Introduction of hsa-miR-520h mimics into PANC-1 cells also resulted in inhibition of cell migration and invasion, and reduction of side population cells. Cell proliferation, cell cycle progression and apoptosis were not affected.Conclusions: We propose that the effects of hsa-miR-520h may be, at least in part, caused by its regulation of ABCG2. Thus, our findings provide a new insight into the function of miRNA in the regulation of ABCG2 expression in pancreatic cancer. Gene therapy using miRNA mimics may therefore be useful as a pancreatic cancer therapy. |
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Keywords: | miRNA ABCG2 migration invasion side population cells |
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