基于顶空气相色谱质谱联用技术的紫雪散挥发性成分研究

[目的] 建立顶空气相色谱质谱联用技术（HSGC-MS）快速鉴定紫雪散中挥发性成分的方法，分析不同批次、不同工艺的紫雪散样品中挥发性成分的差异。[方法] 采用HSGC-MS技术建立紫雪散中挥发性成分的分析方法，利用NIST17标准谱库鉴定紫雪散挥发性成分，并归属各成分的药材来源，运用峰面积归一化法测定各成分的相对含量。[结果] 从紫雪散中共鉴定了46个挥发性成分，包括醇类、酯类、醛类、酮类、酸类、烃类及含氮化合物等，其中最主要成分为麝香酮。不同批次、不同工艺的紫雪散样品中各成分相对含量存在较大差异。[结论] 系统阐释了紫雪散中挥发性成分，为科学评价及有效控制紫雪散质量提供了依据。

Study on volatile components in Zixue Powder by headspace gas chromatography mass spectrometry

[Objective] To establish a rapid analytical method for identifying the volatile components in Zixue Powder by headspace gas chromatography mass spectrometry(HS GC-MS) and evaluate the difference of the volatile components among different batches and different processes of Zixue Powder sample.[Methods] The analytical method of volatile components in Zixue Powder was established by HS GC-MS. The compounds were identified by NIST 17 database retrieval and traced to the herbal sources. The relative content of each compound was determined by peak area normalization method.[Results] The 46 volatile compounds were characterized from Zixue Powder,including alcohols,esters,aldehydes,ketones,acids,hydrocarbons and nitrogenous compounds,and the main compound was muscone. There were great differences in the relative content of the volatile components among different batches and processes of Zixue Powder sample.[Conclusion] The volatile components in Zixue Powder were characterized,which provides a basis for scientific evaluation and effective quality control of Zixue Powder.