猕猴早期胚胎脑的发育与凋亡相关蛋白p53和Bcl-2的表达

为了检测p53、Bcl-2在猕猴胚胎脑中的表达,探讨灵长类胚脑发育过程中生存与凋亡的基因调控规律,我们通过建立猕猴早孕模型,获取早期胚胎,并采用单克隆抗体链菌素亲生物蛋白过氧化酶(SP)免疫组织化学方法,检测了p53和Bcl-2蛋白在猕猴胚胎脑中的表达。结果显示,在猕猴25d、40d和55d胚胎脑均检测到p53和Bcl-2免疫反应阳性神经细胞;随着胎龄的增加,p53和Bcl-2免疫反应阳性表达率逐渐增大。上述结果表明在早期猕猴胚胎脑发育的不同时期均有p53和Bcl-2蛋白表达,这一对相互制约的凋亡相关蛋白在早期胚胎脑发育过程中表达逐渐增加,共同调控脑的发育。

Development of early embryonic brain of rhesus macaque and expression of apoptosis-associated protein p53 and Bcl-2

To detect the expression of p53 and Bcl-2 in embryo brain of rhesus macaque and investigate the role of gene regulation of living and apoptosis during the development of embryo brain in primates.We established the model of early pregnant rhesus macaque,acquired the embryos,and used immunocytochemical histostain-SP method to detect the expression of p53 and Bcl-2 with their corresponding monoclonal antibodies.The results showed that positive cells were stained obviously in 25-day-old,40-day-old and 55-day-old embryonic brains of rhesus macaque,and the expression of p53 and Bcl-2 increased significantly with the increasing of the gestational age.Our data indicate that the expression of p53 and Bcl-2 can be observed in the brain of rhesus monkeys at different period of early embryo development.In addition,the results suggest that the expression of these two correlative apoptosis-associated proteins are increasing gradually during the period of the early embryo development and may control the development of the brain of rhesus macaque cooperatively.