| 与基底细胞癌细胞凋亡相关的分子生物学研究--促凋亡分子Bad的克隆及其pEGFP-C3真核表达载体的构建 |
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| 引用本文： | 胡彬,封兴华,刘芳.与基底细胞癌细胞凋亡相关的分子生物学研究--促凋亡分子Bad的克隆及其pEGFP-C3真核表达载体的构建[J].中国美容医学,2006,15(3):244-246,i0001. |
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| 作者姓名： | 胡彬 封兴华 刘芳 |
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| 作者单位： | 1. 第四军医大学口腔医学院口腔颌面外科,陕西,西安,710032
2. 解放军空军总医院检验科 |
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| 摘 要： | 目的:克隆Bcl-2相关凋亡基因Bad并构建其真核表达载体,探讨其对肿瘤细胞凋亡的诱导作用。方法:采用RT-PCR的方法,扩增Bad基因全长片段。通过基因定向克隆,构建Bad基因的真核表达质粒载体。结果:经酶切鉴定、PCR及DNA序列测定鉴定,Bad基因表达质粒pEGFP-C3载体成功构建。结论:成功克隆Bcl-2相关促凋亡基因Bad并构建其真核表达载体pEGFP-C3-Bad,为进一步研究Bad在人肿瘤细胞系中的促凋亡作用奠定了实验基础。
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| 关 键 词： | 人Bad基因 克隆 绿色荧光蛋白 真核表达载体 基底细胞癌 |
| 文章编号： | 1008-6455（2006）03-0244-03 |
| 收稿时间： | 2005-11-11 |
| 修稿时间： | 2006-01-17 |
Cloning of human Bad gene and construction of its eukaryotic green fluorescent protein expression vector |
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| HU Bin,FENG Xing-hua,LIU Fang.Cloning of human Bad gene and construction of its eukaryotic green fluorescent protein expression vector[J].Chinese Journal of Aesthetic Medicine,2006,15(3):244-246,i0001. |
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| Authors: | HU Bin FENG Xing-hua LIU Fang |
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| Abstract: | Objective To explore the expression of the truncated Bad gene and its pro-apoptotic effect on BCC(basal cell carcinoma ) cells. Methods A full-length human bad gene was cloned by RT-PCR and confirmed by sequence analysis.By deleting the 60 amino acids of N-ter-minal the truncated bad(tbad)gene was attained and then inserted into the eukaryotic expression vector pEGFP-C3. Results pEGFP-C3 containing tBad gene was constructed successfully. Conclusion pEGFP-C3 containing tBad gene was constructed successfully and can be used to explore the function of inducing apoptosis of BCC cells. |
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| Keywords: | human Bad gene clone green fluorescent protein eukaryotic expression vector basal cell carcinoma |
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