BEL-7402 人肝癌细胞分化指标的研究

 目的　探讨肝癌细胞分化的特异指标。方法　用公认的细胞分化诱导剂维甲酸 (RA)、丁酸钠 (SB)和肝细胞毒剂四氯化碳处理 BEL- 740 2人肝癌细胞 ,观察细胞增殖、软琼脂集落形成率、核质比例和各项生化指标的变化。结果　 1 0 μmol/L RA、2 .5mmol/L SB和 1 mmol/L CCl4 均显著抑制肝癌细胞增殖 ,并使其软琼脂集落形成率明显减少 (P<0 .0 5) ,但 RA和 SB处理后 ,细胞核质比例明显减小 (P<0 .0 1或 P<0 .0 5) ,鸟氨酸氨基甲酰转移酶 (OCT)、酪氨酸 -α-酮戊二酸转氨酶 (TAT)和碱性磷酸酶 (ALP)比活力明显升高 ,AFP分泌量和 -谷氨酰转肽酶 ( - GT)比活力明显下降 (P<0 .0 1或 P<0 .0 5) ,而 CCl4 对核质比例和上述生化指标的影响则与 RA和 SB相反。结论　细胞核质比例、AFP分泌量、TAT、OCT和 ALP比活力可作为判断肝癌细胞分化的有用指标 ,其变化与毒性反应有明显的区别。

A Study of the Differentiated Index of BEL-7402 Human Hepatocarcinoma Cell

Objective To explore the specific differentiation indexes ofhepatocarcinoma cells.Methods The BEL-7402 human hepatocarcinoma cell were treated with retinoic acid (RA) and sodium butyrate (SB) (the well known inducer of cell differentiation) or carbon tetrachloride (hepatocytotoxic compound) to observe the differences of cell proliferation,colony-forming ability in semisolid agar culture,nucleocytoplasm ratio and various biochemical indexes.Results When treated with 10μ mol/L RA,2.5mmol/L SB or 1mmol/L CCl4,the proliferation,colony-forming rate in semisolid agar culture of the cells were all reduced.While treated with RA or SB,the nucleocytoplasm ratio of the cells,the specific activities of ornithine carbamoyl transferase(OCT),tyrosine-α-ketoglutarate transaminase (TAT) and alkaline phosphatase (ALP) were significantly increased (P<0.01 or P<0.05),that of γ-glutamyl transpeptidase (γ-GT) and the secretory amount of alpha-fetoprotein(AFP) were significantly decreased (P<0.01 or P <0.05).The effects of CCl4 on the nucleocytoplasm ratio and above biochemical indexes,however,were contrary to that of RA and SB.ConclusionThe nucleocytoplasm ratio,AFP secretion,the specific activities of OCT,TAT and ALP are reliable differentiation indexes of hepatocarcinoma cells,they can distinguish the differentiation effect from the cytotoxic response of hepatocarcinoma cells.