RP-HPLC法测定甘露消渴胶囊中熊果酸的含量

目的:建立以反相高效液相色谱法测定甘露消渴胶囊中熊果酸含量的方法。方法:色谱柱为Hypersil C18(200mm×4.6mm,5μm),流动相为0.05%的磷酸-甲醇(15∶85),流速为1mL.min-1,柱温为40℃,检测波长为207nm。结果:熊果酸进样量在0.96~4.80μg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为94.30%,RSD=1.57%(n=6)。结论:本方法简便、精密度高、重现性好,可用于甘露消渴胶囊的质量控制。

Quantitative Determination of Ursolic Acid in Ganluxiaoke Capsules by RP- HPLC

OBJECTIVE:To establish a RP-HPLC method for the determination of Ursolic acid in Ganluxiaoke capsules.METHODS:The chromatographic column was Hypersil C18(200mm×4.6mm,5μm)at a temperature of 40℃;the mobile phase was 0.05% phosphoric acid-methanol(15∶85)at a flow velocity of 1mL·min-1,and the detection wavelength was set at 207nm.RESULTS:The linear concentration range of Ursolic acid was within 0.96～4.80μg(r=0.999 9).The average recovery rate was 94.30%(RSD=1.57%,n=6).CONCLUSION:This method is simple,precise and reproducible,and it can be used for the quality control of Ganluxiaoke capsules.