| MMP-9在大鼠茄病镰刀菌性角膜炎中的表达 |
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| 引用本文： | 朱江莉,崔红平,英瑛. MMP-9在大鼠茄病镰刀菌性角膜炎中的表达[J]. 同济大学学报(医学版), 2008, 29(6) |
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| 作者姓名： | 朱江莉 崔红平 英瑛 |
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| 作者单位： | 上海市第八人民医院眼科,上海,200235;同济大学附属第十人民医院眼科,上海,200072 |
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| 基金项目： | 上海市科委2008年基础研究重点项目 |
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| 摘 要： | 目的研究基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）在大鼠茄病镰刀菌性角膜炎中的表达，分析MMP-9与真菌性角膜炎发病的关系，为针对抑制MMPs活性治疗真菌性角膜炎提供理论依据。方法角膜划痕法联合角膜接触镜法制作大鼠茄病镰刀菌性角膜炎动物模型；应用实时荧光定量聚合酶链式反应（Real-time polymerase chain reaction，Real-time PCR）和免疫组织化学染色检测MMP-9在mRNA水平和蛋白质水平的表达。结果正常大鼠角膜组织中有MMP-9mRNA的表达，MMP-9分布于角膜上皮层的少数基底细胞；茄病镰刀菌感染角膜后，MMP-9 mRNA表达明显上升，MMP-9主要分布于浸润的炎症细胞。结论MMP-9的表达与真菌性角膜炎角膜病变的严重程度呈正相关，MMP-9在真菌性角膜炎的发病中可能具有重要作用。
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| 关 键 词： | 基质金属蛋白酶-9 真菌性角膜炎 茄病镰刀菌 |
Expression of MMP-9 in Fusarium solani keratitis in rat |
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| ZHU Jiang-li,CUI Hong-ping,YING Ying. Expression of MMP-9 in Fusarium solani keratitis in rat[J]. Journal of Tongji University(Medical Science), 2008, 29(6) |
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| Authors: | ZHU Jiang-li CUI Hong-ping YING Ying |
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| Affiliation: | ZHU Jiang-li~1,CUI Hong-ping~2,YING Ying~2 (1.Dept.of Ophthalmology,Eighth People' s Hospital,Shanghai 200235,China,2.Dept.of Ophthalmology,Tenth People' s Hospital,Tongji University,Shanghai 200072,China) |
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| Abstract: | Objective To study the relationship between fungal keratitis and matrix metalloproteinase-9 (MMP-9) by investigating the expression pattern of MMP-9 in Fusarium solani(F.solani) keratitis rats,so as to offer the possibility of treatment of fungal keratitis by inhibiting MMPs on theory.Methods Experimental model of F.solani keratitis was built by the combination methods of the corneal scarification and hand-made contact lens in rat.Expression of MMP-9 both in mRNA level and protein level were explored by the... |
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| Keywords: | matrix metalloproteinase-9 fungal keratitis Fusarium solani |
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