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Molecular method for identification of Rickettsia species in clinical and environmental samples |
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| Authors: | Jado Isabel Escudero Raquel Gil Horacio Jiménez-Alonso María Isabel Sousa Rita García-Pérez Ana L Rodríguez-Vargas Manuela Lobo Bruno Anda Pedro |
| Affiliation: | Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain. |
| Abstract: | We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens. |
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