异基因小鼠骨髓细胞程序移植后回巢的实验研究

目的探讨骨髓程序移植后移植次数与移植细胞数对回巢的影响.方法采用异基因小鼠骨髓移植模型及流式细胞术,观察不同次数异基因骨髓细胞程序移植后不同时间的回巢状况、不同细胞数异基因骨髓细胞程序移植与单次大剂量移植的回巢状况.结果不同次数移植后各组不同时间阳性回巢细胞数与回巢率均以第3次移植的检测值最高,在共同时间点第4天时均以2次移植组最高(P＜0.05),提示移植后每天都会有回巢发生,但不同移植次数可能在时间上有不同的回巢高峰阶段.不同细胞数异基因骨髓细胞程序移植后,移植细胞数低的移植组与单次大细胞数组比较,回巢细胞数无明显差异,回巢率却远远高于后者(P＜0.01).结果显示在一定剂量范围内多次小剂量移植的回巢效果可能等于或好于单次大剂量移植,且总剂量可能降低至105.结论单次大剂量移植会造成移植干细胞的浪费,在一定细胞量范围内改变移植次数有利于改善移植效果.

Experimental study on homing of bone marrow cells after scheduled transplatation in allogeneic mice

Objective To investigate the effects of transplantation times and number of transplanted cells on homing of these cells after scheduled bone marrow transplantation. Methods An allogeneic mouse model and flow cytometric detection were used to observe homing efficacy of transplantation times and number of cells transplanted. Results After different times of scheduled transplantation,the highest number of homing cells and homing efficiency were both observed after the third time of transplantation in every group,and detecting at same time point (the fourth day) showed that the highest results was obtained in the transplanted group ( P< 0.05),suggesting that there might be different homing patterns after different times of transplantation.After the scheduled transplantation of different number of cells,the number of homing cells of low cell number group was not diffent from that of high number group,but the homing efficiency was higher than the latter ( P< 0.01),suggesting that the homing efficacy of multi-transplantation with low cell number may be equal or superior to that of a single injection of a large number of cells and the accumulated number of cells may be reduced to 10 +5. Conclusion The routine method of transplanting a large number of cells by a single injection may be waste of the stem cells.In a certain range of cell number,transplantation efficiency may be enhanced by changes of transplantation schedule. ;