Development and clinical application of a polymerase chain reaction-based assay for detectingBacteroides ureolyticus |
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| Authors: | Mitsuru Yasuda Takashi Deguchi Masahiro Uno Kouji Tada Hisao Komeda Takayuki Ezaki Yukimichi Kawada |
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| Affiliation: | (1) Department of Urology, Gifu University School of Medicine, 40 Tsukasamachi, 500 Gifu, Japan;(2) Department of Microbiology, Gifu University School of Medicine, 40 Tsukasamachi, 500 Gifu, Japan |
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| Abstract: | We developed a polymerase chain reaction (PCR)-based assay for detectingBacteroides ureolyticus. No DNA fragments hybridizing the internal probe specific forB. ureolyticus were amplified from the DNAs of other bacterial species tested. This assay can detect the amount of DNA corresponding to 18 cells ofB. ureolyticus. Using this assay, we examined urethral swab specimens from men with nongonococcal urethritis (NGU) and asymptomatic men for the presence ofB. ureolyticus. WhileB. ureolyticus was detected in 6 (12.0%) of 50 asymptomatic men, this was not significantly different from the detection rate of 19.3% (22 of 114 specimens) in men with NGU. This study demonstrates that the PCR-based assay can be applied for detectingB. ureolyticus in clinical specimens and suggests that this assay might develop into a useful tool for analyzing the pathogenicity of this organism in various organs. |
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| Keywords: | Bacteroides ureolyticus polymerase chain reaction nongonococcal urethritis |
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