逆针“关元”“三阴交”对去卵巢大鼠下丘脑-垂体-卵巢轴的影响

目的:观察逆针"关元"三阴交"穴对去卵巢大鼠下丘脑-垂体-卵巢(HPO)轴的调节作用,探讨腧穴主治作用的特异性。方法:选取3月龄SD大鼠,设正常对照组(6只)、假手术组(6只)、模型组(6只)、逆针关元组(11只)、逆针三阴交组(11只)、逆针关元续针组(11只)、逆针三阴交续针组(11只),共7组。4组逆针组预先电针15次(隔日1次)后与模型组一同去除大鼠双侧卵巢造模。两组续针组于造模12d后继续电针5次(隔日1次)。采用酶联免疫法测定各组大鼠下丘脑中促性腺激素释放激素(GnRH),垂体中卵泡刺激素(FSH)、促黄体生成激素(LH)和子宫中雌二醇(E2)的含量。结果:与正常组相比,模型组大鼠E2水平显著降低(P<0.05),FSH、LH、GnRH水平明显升高(P<0.01);与模型组相比,各电针组E2水平均有不同程度升高,FSH、LH、GnRH水平均有不同程度降低(P<0.05,P<0.01);但各电针组间比较,差异无统计学意义(P>0.05)。结论:逆针"关元"三阴交"能够良性调节去卵巢大鼠紊乱的HPO轴功能,两穴之间未表现出明显穴位特异性。

Effects of preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) on hypothalamus-pituitary-ovary axis in ovariectomized rats

Objective To observe the effect of preventive-electroacupuncture(PEA) at "Guanyuan"(CV 4) and "San-yinjiao"(SP 6)on hypothalamus-pituitary-ovary(HPO)activities in ovariectomized(OVX) rats,and to compare the specificity of efficacy of acupoints.Methods Sixty-two female SD rats were randomly divided into normal group(n=6),sham-operation(sham) group(n=6),model group(n=6),PEA-Guanyuan(CV 4,PEA-CV 4) group(n=11),PEA-Sanyinjiao(SP 6,PEA-SP 6) group(n=11),complementary(C)-PEA-CV 4(n=11) and C-PEA-SP 6 group(n=11).Climacterium model was duplicated by removing the bilateral ovaries.Before OVX,electroacupuncture(EA,2 Hz/100 Hz,) was applied to bilateral SP 6,CV 4 for 20 min,once every other day for 15 times.For rats of the C-PEA-CV 4 and C-PEA-SP 6 groups and 12 days after the OVX,PEA was applied to CV 4 and SP 6 again,once every other day for 5 times.For rats of the sham group,a little of fat nearby the ovary was removed.Enzyme-linked immunosorbent assay was used to measure the contents of gonadotropin releasing hormone(GnRH) in the hypothalamus,follicule-stimulating hormone(FSH) and luteinizing hormone(LH) in the hypophysis,and estrogen(E2) in the uterus.Results In comparison with the normal group,uterus E2 content was decreased significantly(P<0.05),and pituitary FSH and LH contents and hypothalamic GnRH content were increased obviously(P<0.01) in the model group.Compared with the model group,uterus E2 levels in the PEA-CV 4 and C-PEA-CV 4 groups were increased apparently(P<0.05),while both pituitary FSH and hypothalamic GnRH levels in the PEA-CV 4,C-PEA-CV 4,PEA-SP 6 and C-PEA-SP 6 groups,and pituitary LH levels in the C-PEA-CV 4 and PEA-SP 6 groups were down-regulated markedly(P<0.05,P<0.01),suggesting an improvement of the HPO axis activity following PEA and C-PEA.No significant differences were found between the normal and sham groups in uterus E2,pituitary FSH and LH,and hypothalamic GnRH contents,and between the model and PEA-SP 6 groups,and between the model and C-PEA-SP 6 groups in uterus E2 levels,and between the model and PEA-CV 4 groups,and between the model and C-PEA-SP 6 groups in pituitary LH levels(P>0.05).Conclusion Preventive EA of "Guanyuan"(CV 4) and "Sanyinjiao"(SP 6) can function in up-regulating uterus E2 level,and down-regulating pituitary FSH and LH contents and hypothalamic GnRH level in OVX rats,which may contribute to its efficacy in relieving climacteric syndrome in clinic.