磺胺嘧啶亲人鼻咽癌CNE2特性的实验研究

目的 验证磺胺嘧啶的亲人鼻咽癌CNE2特性。方法 磺胺嘧啶通过双功能螯合剂二乙撑三氨五乙酸标记放射性核素99mTc，放射性纸层析测定标记率。制作荷人鼻咽癌CNE2裸鼠动物模型，将放射性标记物通过尾静脉注入动物体内，6h后测定各主要脏器及肿瘤组织的放射性，计算肿瘤组织/非肿瘤组织（T/NT）放射性比。结果 在生理盐水层析液中，标记率为90.41%；在正丁醇：乙醇：水层析液中，标记率为90.63%。99mTc标记的磺胺嘧啶经尾静脉注射6h后在荷人CNE2鼻咽癌裸鼠体内的分布与游离99mTc对照组比较，肝脏、脾脏、骨髓的放射性明显减少（P﹤0.05），肿瘤组织的放射性明显增加（P﹤0.01），除了肾脏外，肿瘤组织的放射性高于其他组织、器官，肿瘤组织/非肿瘤组织比值在3.27：1 - 4.53：1。结论 磺胺嘧啶具有亲人鼻咽癌CNE2的特性，有作为载体对鼻咽癌进行靶向治疗的潜能

Experiment study on the selective affinity of sulfadiazine to human nasopharyngeal cell line CNE2

Objective The aim of this investigation was to testify the selective affinity of sulfadiazine (SF) to tumor cells. Methods SFPEG (polymer of SF and Ploy(ethylene glycol)) was labeled by 99mTc via diethylenetriamine pentoacetic acid (DTPA). Labeling efficiency was determined by paper chromatography. Nude mice bearing human nasopharyngeal cell line CNE2 were used to evaluate the biodistribution of SF. The tumor bearing mice were sacrificed at 6h after 99mTc labeled SF was injected though tail-vein. Tumor, liver, spleen, kidney, bone marrow, muscle and heart were obtained from the tumor bearing mice and the radioactivity present on each sample was measured by liquid scintillation counting. Tumor to non-tumor ratios were calculated. Results Labeling efficiency was over 90% as determined by paper chromatography in two different mobile phase. Radioactivity of the tumor tissue was increased significantly at 6h post injection and was higher than the non-tumor tissues except kidney. T/NT ratios ranged from 3.27:1 to 4.53:1.Conclusion Sulfadiazine can be concentrated by human nasopharyngeal cell line CNE2, and Sulfadiazine may be a promising carrier for tumor targted therapy.