| 细胞色素P450 2D6等位基因CYP2D6C的特异扩增法测定 |
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| 引用本文： | 陈枢青,孙红颖,赵鲁杭,Peter J Wedlund. 细胞色素P450 2D6等位基因CYP2D6C的特异扩增法测定[J]. 中国药理学与毒理学杂志, 1999, 13(2): 149-151 |
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| 作者姓名： | 陈枢青 孙红颖 赵鲁杭 Peter J Wedlund |
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| 作者单位： | 浙江医科大学生物化学教研室 |
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| 摘 要： | 细胞色素Ｐ４５０２Ｄ６（ＣＹＰ２Ｄ６）第２７０３－５位ＡＡＧ缺失造成表达产物第２８１位赖氨酸缺失的等位基因称为ＣＹＰ２Ｄ６Ｃ．利用等位基因特异扩增法（ＡＳＡ）原理，建立了ＡＳＡ－ＰＣＲ测定ＣＹＰ２Ｄ６的方法．经３９６例测定，说明本法快捷，污染少．测定结果显示ＣＹＰ２Ｄ６Ｃ不属于ＣＹＰ２Ｄ６酶缺陷等位基因，表型仍为快代谢．
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| 关 键 词： | 细胞色素P450 基因型 等位基因 基因扩增 |
| 收稿时间： | 1998-01-14 |
Detection of cytochrome P4502D6 allele CYP2D6C with allele specific amplification |
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| Peter J Wedlund. Detection of cytochrome P4502D6 allele CYP2D6C with allele specific amplification[J]. Chinese Journal of Pharmacology and Toxicology, 1999, 13(2): 149-151 |
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| Authors: | Peter J Wedlund |
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| Affiliation: | (Department of Biochemistry，Zhejiang Medical University， Hangzhou 310006； 2. College of Pharmacy, University of Kentucky, Lexington KY 40536 USA) |
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| Abstract: | An allele specific amplification based PCR method was built up for the detection of CYP2D6C allele which is a 2703 5 AAG deletion on CYP2D6 gene and results in a 281 position lysine deletion. This method was employed to genotyping 396 subjects and proved to be simple, quick, accurate and less contamination. The results show that the CYP2D6C was not an enzyme deficient allele. |
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| Keywords: | cytochrome P450 genotype alleles gene amplification |
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