| Abstract: | Localized delivery of antisense oligonucleotides directed against cell cycle regulatory proteins has been proposed as a means to prevent restenosis after angioplasty. To test whether single endoluminal delivery of a combination of proliferating cell nuclear antigen (PCNA) and cell-division cycle 2 kinase (cdc2) antisense might affect restenosis, we delivered 2 ml of lipid-complexed PCNA/cdc2 antisense oligomers (1.35 mg) to the coronary arteries of pigs after balloon overstretch angioplasty (AS group) and performed planimetric histomorphometry on arterial sections of the tissue, harvested at 4 wk. Compared with controls receiving 3′–5′ reversed sequence oligomers (REV group), there were no differences in absolute intimal area (AS 1.36 ± 0.08 mm2, REV 1.23 ± 0.10 mm2, P = NS), intimal area normalized to extent of injury (AS 0.67 ± 0.03, REV 0.77 ± 0.10, P = NS), or vessel perimeter (AS 7.72 ± 0.19 mm, REV 7.36 ± 0.22 mm, P = NS). We conclude that single endoluminal delivery of antisense against key cell cycle regulatory proteins does not affect neointima formation or vessel size in this model of restenosis. Cathet. Cardiovasc. Diagn. 41:348–353, 1997. © 1997 Wiley-Liss, Inc. |
