| Abstract: | The developmental profile of the neurofilament (NF) triplet proteins, α-internexin and peripherin in cultured dorsal root ganglion neurons from gestation day 15 rat embryos was determined by Western blot analysis. At the outset (day 0 in culture), the neurons contained mostly α-internexin. A significant increase in peripherin levels was seen at days 1–2, in the mid-sized (NFM) and low molecular weight (NFL) NF subunits at days 2–3, and in the high molecular weight (NFH) NF subunit at days 5–6. Immunofluorescence microscopy showed that the five intermediate filament proteins were co-localized in all neuronal cell bodies and neurites. Analysis of Triton X-100 extracts from okadaic acid-treated dorsal root ganglion cultures revealed that peripherin and α-internexin followed the same fragmentation pattern observed with NFs. Interactions between the various neuronal intermediate filament proteins in these extracts were assessed by immunoprecipitation under native conditions using antibodies specific for the individual proteins. Co-immunoprecipitation of NFH with NFL, NFM with NFL, NFM with α-internexin, and α-internexin with peripherin demonstrated that the intermediate filament cytoskeleton in cultured sensory neurons is a highly integrated structure. J. Neurosci. Res. 47:300–310, 1997. © 1997 Wiley-Liss, Inc. |
