维拉帕米对烧伤后大鼠心肌肌球蛋白异构体转换的影响

目的　探讨烧伤后心肌细胞肌球蛋白异构体转换及维拉帕米对其影响。方法　 110只大鼠随机分为 4组 :单烧组 ( 3 0 %总体表面积Ⅲ度烧伤 ) ;维拉帕米组 (维拉帕米溶液 4mg·kg- 1 ) ;补液组 (乳酸林格氏液 4ml·kg- 1 ·1%烧伤面积 - 1 )和对照组。通过聚丙烯酰胺凝胶电泳 (SDS PAGE)观察肌球蛋白异构体的改变。结果　烧伤后 12h(维拉帕米组 2 4h)开始肌球蛋白由V1向V3转换 ,与对照组比较 ,3个实验组存在显著性差异 (P <0 .0 5 ) ,维拉帕米组 ( 2 4、72h)与单烧组和补液组之间存在显著性差异 (P <0 .0 5 ) ,而单烧组和补液组之间无显著性差异 (P >0 .0 5 )。结论　烧伤引起心肌收缩蛋白 肌球蛋白异构体V1向V3转换 ,维拉帕米部分阻止肌球蛋白异构体的转换

Effect of verapamil on the transformation of myosin isoforms in rat myocardial cells after burn

Objective To study the transformation of myosin isoforms and the effect of verapamil on the transformation after burn. Methods Wistar rats were randomly divided in four groups: the burn group(BG, inflicted with 30% TBS third degree burn), the verapamil group(VG), the fluid replacement group(RG) and the control group(CG). The transformation of myosin was observed by SDS PAGE. Results The transformation of myosin from V1 to V3 in three experimental groups was found at the 12 th hour(at 24 th hour in verapamil group)after burn, which was significantly different from that in the control group( P <0.05). Verapamil could evidently reduce the myosin transformation from V1 to V3( P <0.05). There was no significant difference from the burn group and the fluid replacement group( P >0.05). Conclusion Burn can induce the transformation of myocardial contractile protein myosin isoforms from V1 to V3, which can be partially prevented by verapamil.