| 守宫硫酸多糖对肝癌SMMC-7721细胞分化和增殖的影响 |
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| 引用本文： | 辛亮,吴雄志,谢广茹. 守宫硫酸多糖对肝癌SMMC-7721细胞分化和增殖的影响[J]. 天津医药, 2011, 39(12): 1123-1126. DOI: 10.3969/j.issn.0253-9896.2011.12.011 |
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| 作者姓名： | 辛亮 吴雄志 谢广茹 |
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| 作者单位： | 天津市“肿瘤防治”重点实验室, 天津医科大学附属肿瘤医院高级病房,300060 |
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| 摘 要： | 目的:研究守宫硫酸多糖(Gepsin)对肝癌SMMC-7721细胞分化和增殖的影响,并进一步探讨其可能的机制。方法:将不同浓度的Gepsin加入对数生长的SMMC-7721细胞进行培养,在不同的时间利用台盼兰染色观察细胞的增殖情况。同时,收集细胞培养上清液,利用联合放免法,以溴钾酚绿法分别观察Gepsin对甲胎蛋白(AFP)、白蛋白(ALB)分泌的影响;利用酶联免疫吸附试验法观察Gepsin对转化生长因子(TGF)-β1、血管内皮生长因子(VEGF)分泌的影响;利用光镜观察Gepsin对SMMC-7721细胞形态的影响。结果:随着处理组Gepsin的浓度升高,SMMC-7721细胞的增殖明显受到抑制;而对细胞活率无明显影响。Gepsin可增加培养上清中的ALB分泌量,降低AFP分泌量。光学显微镜下可见加入Gepsin后细胞的形态由圆形变为纺锤形。Gepsin对培养上清分泌的VEGF无影响,但可使TGF-β1增加。结论:Gepsin可明显抑制肝癌细胞SMMC-7721的增殖,诱导SMMC-7721细胞分化。其机制可能是通过上调TGF-β1来诱导肝癌细胞分化实现的。
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| 关 键 词： | 白蛋白类 |
Effects of Gekko Sulfated Polysaccharide on the Proliferation and Differentiation in Hepatic Cancer Cell Line SMMC-7721 |
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| XIN Liang,WU Xiongzhi,XIE Guangru. Effects of Gekko Sulfated Polysaccharide on the Proliferation and Differentiation in Hepatic Cancer Cell Line SMMC-7721[J]. Tianjin Medical Journal, 2011, 39(12): 1123-1126. DOI: 10.3969/j.issn.0253-9896.2011.12.011 |
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| Authors: | XIN Liang WU Xiongzhi XIE Guangru |
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| Affiliation: | XIN Liang,WU Xiongzhi,XIE Guangru Cancer Institute and Hospital of Tianjin Medical University,Key Laboratory of Cancer Prevention and Therapy,Tianjin 300060,China |
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| Abstract: | Objective: To investigate the effect of Gekko sulfated polysaccharide (Gepsin) on the proliferation and differentiation in hepatic cancer cell line SMMC-7721. Methods: SMMC-7721 cells were cultured in RPM-1640 medium with different concentrations of Gepsin. Trypan blue stain was used to determine the cell proliferations in different time points. The cell culture supernatant was collected to observe the effect of Gepsin on alpha-fetoprotein (AFP) and albumin (ALB) by bromocresol green-immunology. Levels of t... |
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| Keywords: | GEKKO SWINHOANA polysaccharides cell line tumor liver neoplasms carcinoma alpha-fetoproteins albumins transforming growth factor beta1 vascular endothelial growth factors |
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