| 缺血后处理对大鼠心肌细胞凋亡及Bcl-xl/s的影响 |
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| 引用本文： | 董福强,田轶魁,魏民新,张鑫. 缺血后处理对大鼠心肌细胞凋亡及Bcl-xl/s的影响[J]. 天津医药, 2011, 39(12): 1130-1132,1194. DOI: 10.3969/j.issn.0253-9896.2011.12.013 |
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| 作者姓名： | 董福强 田轶魁 魏民新 张鑫 |
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| 作者单位： | 1. 300052,天津医科大学总医院心血管外科
2. 300052,天津医科大学总医院内分泌实验室 |
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| 基金项目： | 国家自然科学基金资助项目，天津市应用基础及前沿技术研究计划项目，天津医科大学科学基金资助项目 |
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| 摘 要： | 目的:从Bcl-xl/s基因和蛋白水平探讨缺血后处理抗大鼠心肌细胞凋亡的机制。方法:18只健康Wistar大鼠随机分为假手术(Sham)组、缺血再灌注损伤(I/R)组、缺血后处理(PostC)组,每组6只。采用原位缺口末端标记(TUNEL)检测大鼠心肌细胞凋亡指数;免疫组化法检测BcL-xl/s蛋白表达水平;荧光实时定量PCR法检测心肌组织Bcl-xl/s基因表达水平。结果:与I/R组相比,PostC组再灌注后心肌细胞凋亡指数明显降低,PostC组Bcl-xl蛋白及mRNA表达水平明显上调,Bcl-xs蛋白及mRNA表达水平明显下调。结论:PostC能显著减少缺血再灌注后心肌细胞凋亡,凋亡相关基因Bcl-xl、Bcl-xsmRNA及蛋白参与了由缺血再灌注诱导的心肌细胞凋亡。
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| 关 键 词： | 逆转录聚合酶链反应 |
Effects of Ischemic Postconditioning on Cardiomycyte Apoptosis and Expression of Bcl-xl/s in Rats |
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| DONG Fuqiang,TIAN Yikui,WEI Minxin,ZHANG Xin. Effects of Ischemic Postconditioning on Cardiomycyte Apoptosis and Expression of Bcl-xl/s in Rats[J]. Tianjin Medical Journal, 2011, 39(12): 1130-1132,1194. DOI: 10.3969/j.issn.0253-9896.2011.12.013 |
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| Authors: | DONG Fuqiang TIAN Yikui WEI Minxin ZHANG Xin |
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| Affiliation: | DONG Fuqiang,TIAN Yikui,WEI Minxin,ZHANG Xin Department of Cardiovascular Surgery,General Hospital of Tianjin Medical University,Tianjin 300052,China |
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| Abstract: | Objective:To study the mechanism of anti-apoptosis and changed expression of Bcl-xl/s in ischemic postconditioning (PostC) in rats. Methods: Eighteen healthy adult male Wistar rats were divided into three groups including Sham group, ischemia-reperfusion (I/R) group and PostC group (n=6 for each). TUNEL staining was used to analyse the myocardial apoptotic index. Immunohistochemisty was used to detect the protein level of Bcl-xl/s. The expressions of Bcl-xl mRNA and Bcl-xs mRNA were determined by real-time ... |
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