罗格列酮对OLETF鼠皮肤组织及其晚期糖基化产物的影响

目的：探讨罗格列酮对OLETF鼠皮肤组织及其晚期糖基化产物（AGEs）的影响和机制。方法：OLETF雄性大鼠20只．30周时共有成模的2型糖尿病（T2DM）OLETF大鼠12只，随机分为罗格列酮组和模型组（每组6只），对照组为LETO鼠（8只）。各组均于给药12周后同时采集背部皮肤组织，HE染色观察皮肤的组织学变化及测量表皮和真皮的厚度，Masson三色染色观察胶原纤维的变化，免疫组织化学染色观察AGEs的蓄积程度及变化。结果：模型组大鼠表皮组织变薄，表皮细胞欠清晰。部分表皮缺乏复层排列；真皮层亦变薄，胶原纤维纤细、排列紊乱，部分胶原可见肿胀、变性。罗格列酮组大鼠皮肤表皮层较清晰，复层排列接近对照组，真皮胶原丰富，表皮与真皮厚度明显增加，与对照组无明显差异。模型组大鼠皮肤组织AGEs明显蓄积在真皮基质和细胞中，阳性表达明显高于罗格列酮组和对照组。结论：2型糖尿病在皮肤未受损伤时已发生组织学变化，AGEs可能是导致这种变化的机制之一，而罗格列酮可有效减轻这种损害的发生。

The effect of Rosiglitazone on the skin and AGEs in OLETF rats

Objective: To research the effect and mechanism of rosiglitazone on the skin and AGEs in OLETF rats.Methods: 20 male OLETF rats were given high-calorie diet for 30 weeks to set up the T2DM.At the 30th week,12 male induced OLETF rats were randomized into two groups: model group and rosiglitazone group 8 LETO rats were used as controls.The shaved skin specimens from the back of rats in three groups were collected.Skin AGEs concentrations were assessed by immunohistochemistry assay.Hematoxylin-eosin dye was us...