| CsA对缺血再灌注心肌高能磷酸化合物代谢的影响 |
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| 引用本文： | 樊燕燕,甄江涛,朱宁,赵晓丽,曹颖,王丽艳,于公元,张艳君. CsA对缺血再灌注心肌高能磷酸化合物代谢的影响[J]. 天津医科大学学报, 2011, 17(4): 464-466 |
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| 作者姓名： | 樊燕燕 甄江涛 朱宁 赵晓丽 曹颖 王丽艳 于公元 张艳君 |
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| 作者单位： | 天津医科大学生物化学教研室,天津,300070 |
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| 摘 要： | 目的：研究环孢素A（CsA）对大鼠心肌缺血再灌注损伤能量代谢的影响。方法：建立大鼠离体心肌缺血再灌注模型，测定心肌梗死面积，使用高效液相色谱（HPLC）法测定心肌组织中高能磷酸化合物ATP、ADP和AMP的含量，测定心肌组织Na^＋-K^＋-ATPase以及乳酸脱氢酶（LDH）活性，观察环孢素A（0．1mg/mL）的保护作用。结果：环孢素A能显著降低缺血再灌注心脏灌流液的LDH水平，提高心肌组织ATP含量和能量物质的储备，降低心肌组织ATPase活性。结论：环孢素A对大鼠心肌缺血再灌注损伤有一定的保护作用。其作用机制可能与增加缺血区心肌能量物质含量，降低ATPase活性，改善能量代谢障碍有关。
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| 关 键 词： | 环孢素A 缺血再灌注 能量代谢 大鼠 |
Effect of ciclosporin A on high energy phosphates in rats with myocardial ischemia-reperfusion injury |
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| FAN Yan-yan,ZHEN Jiang-tao,ZHU Ning,ZHAO Xiao-li,CAO Ying,WANG Li-yan,YU Gong-yuan,ZHANG Yan-jun. Effect of ciclosporin A on high energy phosphates in rats with myocardial ischemia-reperfusion injury[J]. Journal of Tianjin Medical University, 2011, 17(4): 464-466 |
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| Authors: | FAN Yan-yan ZHEN Jiang-tao ZHU Ning ZHAO Xiao-li CAO Ying WANG Li-yan YU Gong-yuan ZHANG Yan-jun |
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| Affiliation: | FAN Yan-yan,ZHEN Jiang-tao,ZHU Ning,ZHAO Xiao-li,CAO Ying,WANG Li-yan,YU Gong-yuan,ZHANG Yan-jun(Department of Biochemistry,Tianjin Medical University,Tianjin 300070,China) |
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| Abstract: | Objective: To study effect of ciclosporin A on energy metabolism in rats with myocardial ischemia-reperfusion injury.Methods: The ischemia-reperfusion model of rats was established by coronary artery ligation.The high energy phosphates(ATP,ADP and AMP) levels in myocardial tissue were measured by HPLC.The activities of the effluent of coronary artery lactate dehydrogenase(LDH),supernatant ATPases and myocardial infarction size in myocardial tissue were assayed.Results:Ciclosporin A reduced the effluent of c... |
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| Keywords: | Ciclosporin A Ischemia reperfusion Energy metabolism Rat |
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