高效液相色谱法测定兔玻璃体内美罗培南浓度的方法研究

目的:建立一种反相高效液相色谱法--紫外线检测法,测定玻璃体内的美罗培南浓度。方法:应用Agilent1100系列型高效液相色谱仪,色谱柱为EclipseXDB-C8,流动相为乙腈-水(乙腈:水=10∶90),检测波长298nm,参比波长:400nm,加入乙腈自然沉淀,提取剂为乙腈,内标物为对乙酰氨基酚,流速为0.8mL/min。结果:本方法测定的线性范围为0.05～2.5mg/mL,最低检测浓度是0.05mg/mL,回收率为98.93%,日内精密度≤3.03%,日间精密度≤5.49%。结论:该方法简单,准确,适合于玻璃体腔注射美罗培南的药物浓度测量。

RP-HPLC Method for Determination of Meropenem in Rabbit Vitreum

Objective To develop a RP-HPLC method for determining vitreous concentration of meropenem in rabbit. Methods The propofol was separated on a Eclipse XDB-C8 column,the mobile phase was a mixture of acetonitrile and water(10∶90).UV detection wavelength was 298 nm;Reference wavelength was 400 nm.Sample preparation was simply a deproteinsation process with acetonitrile. The meropenem was extracted by the acetonitrile.Internal standard was acetaminophen.Flow rate was 0.8 mL/min.Results Excellent liner relationsh...