| HPLC法测定有机化学实验用4种茶中的咖啡因 |
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| 引用本文： | 苏超,赵东升,匡添,李新霞,高茜,兰欢,苏丹,巴哈尔古丽·卡哈尔,支玲.HPLC法测定有机化学实验用4种茶中的咖啡因[J].新疆医科大学学报,2011,34(5):501-503. |
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| 作者姓名： | 苏超 赵东升 匡添 李新霞 高茜 兰欢 苏丹 巴哈尔古丽·卡哈尔 支玲 |
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| 作者单位： | 1. 新疆医科大学药学院,新疆,乌鲁木齐,830011
2. 新疆医科大学药化有机教研室,新疆,乌鲁木齐,830011 |
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| 基金项目： | 新疆医科大学大学生创新性实验计划项目 |
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| 摘 要： | 目的 HPLC法测定学生有机化学实验用的四种茶叶中的咖啡因的含量。方法 4种茶叶分别用乙醇(95%)经索氏提取器提取,用HPLC法测定咖啡因含量后与学生实验结果进行比较。结果学生提取咖啡因含量:绿茶1为0.26%、绿茶2为0.29%、红茶为0.29%、砖茶为0.17%,HPLC测定结果:绿茶1为1.87%、绿茶2为2.73%、红茶为2.36%、砖茶为0.73%,提取率为10.62%～23.29%,绿茶2、红茶含量最高、砖茶含量较低,学生提取结果与HPLC法测定结果的结论一致。结论学生实验获得的咖啡因纯度较高,说明升华法是纯化咖啡因的有效方法,但提取率低。影响提取率的因素很多,标准规范的实验操作是学生实验提高收率的保证条件之一。
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| 关 键 词： | 咖啡因 高效液相色谱 茶叶 分离提取 含量测定 |
Determine the extracting caffeine from four type teas by HPLC in organic chemistry experiment |
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| SU Chao,ZHAO Dong-sheng,KUANG Tian,et al.Determine the extracting caffeine from four type teas by HPLC in organic chemistry experiment[J].Journal of Xinjiang Medical University,2011,34(5):501-503. |
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| Authors: | SU Chao ZHAO Dong-sheng KUANG Tian |
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| Institution: | SU Chao,ZHAO Dong-sheng,KUANG Tian,et al(Pharmacy College,Xinjaing Medical University,Urumqi 830011,China) |
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| Abstract: | Objective To evaluate the caffeine content of extract from four type teas in student organic chemistry experiment.Methods The teas were extracted with ethanol(95%) by Soxhlet extractor.The content of caffeine was assayed by high performance liquid chromatography(HPLC).Results The extraction ratio of the caffeine fulfilled by students are: green tea 1 has 0.26%,green tea 2 has 0.29%,black tea has 0.29% and brick tea has 0.17%.The content of the caffeine determined by HPLC are: green tea 1 has 1.87%,green tea... |
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| Keywords: | caffeine high performance liquid chromatography(HPLC) tea isolate extract assaying |
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