壬基酚和镉离子在体外对小鼠精子顶体反应的影响

目的:研究壬基酚和镉离子在体外对小鼠精子顶体反应(AR)的影响。方法:从小鼠的输精管获得精子,体外培养使精子获能,加30μmol/L的A23187诱导精子顶体反应,然后使用不同浓度的壬基酚(10、20、30、60、100μmol/L),或者镉离子(500、2500、5000μmol/L)处理,对照组使用相应的载体溶剂处理。用FITC-PSA荧光染色法分析精子顶体反应。结果:当壬基酚浓度<30μmol/L时,小鼠精子顶体反应率与对照组比较没有显著差异(P>0.05),而当壬基酚浓度>60μmol/L时能够显著地抑制小鼠精子顶体反应发生率(P<0.01),并且观察到精子存活率随着壬基酚浓度增加而降低。与壬基酚作用不同,用镉离子对小鼠精子进行处理,在所选浓度内(500~5 000μmol/L)均对精子顶体反应无显著影响(P>0.05),且精子存活率与镉离子浓度变化无关。结论:壬基酚与镉离子对小鼠精子发生的作用是通过不同的途径来实现的,前者可以直接抑制顶体反应,而后者则与精子顶体反应无关。

Effects of nonylphenol and cadmium on sperm acrosome reaction in vitro in mice

Objective： To study the effects of nonylphenol and cadmium on acrosome reaction in vitro in mouse spermatozoa. Methods： Sperm were collected from the vas deferens of mice, capacitated in vitro and stimulated with A23187 at 30μmol/L to induce acrosome reaction. Then the sperm suspension was treated with nonylphenol at 10, 20, 30, 60 and 100μmol/L or cadmium at 500, 2500 and 5 000 μmol/L, and the control group treated with the carrier solvent. Aerosome reaction of the sperm was analyzed by FITC- PSA staining. Results ： Compared with the control group, nonylphenol significantly inhibited acrosome reaction at the concentration of 〉 60 μmol/L （P 〈 0. 01 ）, but not at 〈 30μmol/L （P 〉 0. 05 ）, and the sperm survival rate was reduced with increased concentration of nonylphenol. However, cadmium exhibited no significant influence on either acrosome reaction （P 〉 0.05 ） or sperm survival rate at 500 - 5 000 μmol/L. Conclusion ： Nonylphenol and cadmium affect the spermatogenesis of mice in different ways ; the former directly inhibits sperm acrosome reaction, while the latter has no direct effect on it.