| 氧化修饰低密度脂蛋白的蛋白组份可引起巨噬细胞线粒体膜的损伤 |
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| 引用本文： | 刘尚喜,周玫,陈瑗,孙曼霁. 氧化修饰低密度脂蛋白的蛋白组份可引起巨噬细胞线粒体膜的损伤[J]. 南方医科大学学报, 1996, 0(4) |
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| 作者姓名： | 刘尚喜 周玫 陈瑗 孙曼霁 |
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| 作者单位： | 军事医学科学院毒物药物研究所 |
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| 摘 要： | 采用ＡＣＡＳ５７０测定小鼠腹腔巨噬细胞线粒体膜电位，观察了氧化修饰低密度脂蛋白（Ｏｘ－ＬＤＬ）对巨噬细胞线粒体膜的损伤及其不同组份（ｍｏｉｅｔｙ）在致损伤中的作用。结果显示Ｏｘ－ＬＤＬ可使巨噬细胞线位体膜产生损伤，用ＧＳＨＰｘ模拟物ｅｂｓｅｌｅｎ清除Ｏｘ－ＬＤＬ上的脂氢过氧化物（ＬＯＯＨ）可使损伤减轻２０％。单独的脂质组份虽然含有大量的ＬＯＯＨ，但对巨噬细胞线粒体膜电位无显著影响，而单独的蛋白组份则可引起线粒体膜电位下降，其降低程度与Ｏｘ－ＬＤＬ清除ＬＯＯＨ后的降低程度相当，说明Ｏｘ－ＬＤＬ清除ＬＯＯＨ后对线粒体膜的损伤可能是由修饰的载脂蛋白Ｂ（ａｐｏＢ）产生的。
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| 关 键 词： | 氧化修饰低密度脂蛋白；巨噬细胞；线粒体膜电位；脂氢过氧化物；载脂蛋白B |
The protein moiety in oxidized low density lipoprotein may cause in-jury to mitochondrial membrane of mouse peritoneal macrophages |
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| Liu Shangxi, Zhou Mei, Chen Yuan, Sun Manji. The protein moiety in oxidized low density lipoprotein may cause in-jury to mitochondrial membrane of mouse peritoneal macrophages[J]. Journal of Southern Medical University, 1996, 0(4) |
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| Authors: | Liu Shangxi Zhou Mei Chen Yuan Sun Manji |
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| Abstract: | The role of lipid hydroperoxides(LOOH),the lipid and protein moiety in Ox-LDL in the injuryto macrophage mitochonodrial membrane induced by Ox-LDL was observed by measuring mitochondrialmernbrane potential on ACAS570.The results showed that the mitochondrial membrane potential de-creased when macrophages were treated by Ox-LDL,If LOOH in Ox-LDL was precleared by ebselen plusGSH,the decreased mitochondrial membrane potential could be recovered by about 20%. The lipid moietyalone although containing a large amount of LOOH had no significant effect on mitochondrial membranepotential.The protein moiety alone could make the membrane potential decrease,and the degree of the de-crease was equivalent with that of Ox-LDL in which LOOH was cleared by ebselen plus GSH. |
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| Keywords: | oxidatively modified low density lipoprotein macrophages mitochondrial membrane poten-tial lipid hydroperoxides apoB |
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