模拟失重大鼠动脉血管紧张素受体基因表达的研究

目的：探讨模拟失重下大鼠不同部位动脉血管紧张素受体mRNA表达是否发生变化。方法：大鼠被随机分为模拟失重组（SUS）与同步对照组（CON），采用尾部悬吊大鼠模型模拟失重影响。用逆转录-聚合酶链式反庆（RT-PCR）技术观察比较模拟失重下大鼠不同部位动脉血管紧张素两型受体基因表达的变化。结果：大鼠颈总动脉，腹主动脉及股动脉组织均有血管紧张素Ⅱ(AngⅡ)的AT1a，AT1b及AT2受体mRNA的表达；而基底动脉组织则只有AT1a和AT1bmRNA的表达。模拟失重4周大鼠颈总动脉、腹主动脉、股动脉及基底动脉组织AngⅡ的AT1a、AT1b和AT2受体mRNA的表达，较对照大鼠均无显著变化。结论：模拟失重引起大鼠动脉血管结构与功能的变化可能不是通过血管紧张素受体的变化实现的。

A Study on Gene Expression of Angiotensin Receptors in Arteries from Tail-suspended Rats

Objective. To investigate whether simulated microgravity correction of microgravtiy] can result in alterations of mRNA expression of angiotensin receptors in rat arteries. Method. Rats were divided into simulated microgravity group and synchronous control group. Simulated microgravity rats were subjected to tail-suspension for 4-wk to simulate the effect of weightlessness. Result. Results from RT-PCR demonstrated that mRNAs of angiotensin II receptors, AT1a, AT1b, and AT2 were expressed in carotid arteries, abdominal aorta and femoral arteries, whereas only mRNAs of AT1a and AT1b were expressed in basilar arteries. There were no significant differences between the simulated-microgravity and control rats in the expression of mRNAs of AT1a, AT1b, and AT2 in these arteries. Conclusion. Angiotensin II receptors may play no regulatory role in the occurrence of the differentiated vascular adaptation during simulated microgravity.