| 急性淋巴细胞白血病8号染色体三体 |
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| 引用本文： | 肖冰,李建勇,潘金兰,李丽,薛永权,吴亚芳.急性淋巴细胞白血病8号染色体三体[J].江苏医药,2005,31(4):241-243,i002. |
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| 作者姓名： | 肖冰 李建勇 潘金兰 李丽 薛永权 吴亚芳 |
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| 作者单位： | 210029,南京医科大学第一附属医院血液科;苏州大学附属第一医院 |
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| 基金项目： | 江苏省135工程医学重点人才基金(RC2002044) |
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| 摘 要： | 目的探讨急性淋巴细胞白血病(ALL)中8号染色体三体(8三体)的发生率。方法对87例ALL和8例正常对照骨髓细胞进行经典的细胞遗传学(CC)及红色荧光素Spectrum Red标记的8号染色体着丝粒α卫星特异DNA探针间期荧光原位杂交技术(FISH)分析。结果87例ALL中14例FISH检测为8三体，占16．09％，5例CC检测结果为8三体(5．75％，5／87)；FISH还发现8三体／四体嵌合体1例，而CC仅检测到8四体。结论FISH检测8三体的敏感性高于常规核型分析，在小克隆检测方面有其优越性。
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| 关 键 词： | 急性淋巴细胞白血病 8号染色体 细胞遗传学 荧光原位杂交 |
A study of trisomy 8 in acute lymphoblastic leukemia |
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| XIAO Bing,LI Jianyong,PAN Jinlan,et al..A study of trisomy 8 in acute lymphoblastic leukemia[J].Jiangsu Medical Journal,2005,31(4):241-243,i002. |
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| Authors: | XIAO Bing LI Jianyong PAN Jinlan |
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| Institution: | XIAO Bing,LI Jianyong,PAN Jinlan,et al. ~Department of Hematology,First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,CHINA |
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| Abstract: | Objective To investigate the frequency of trisomy 8 in acute lymphoblastic leukemia(ALL). Methods The bone marrow cells of 87 cases of ALL and 8 normal controls were studied by the techniques of conventional cytogenetics(CC) and interphase fluorescence in situ hybridization (FISH) with SpectrumRed labeled chromosome 8 centrome specific probe . Results Among 87 ALL cases,14 (16.0%) were trisomy 8 by FISH, however,only 5 (5.8%) cases were trisomy 8 by CC. Moreover, FISH detected 1 case with trisomy/tetrasomy 8,CC showed only tetrasomy. Conclusion Interphase FISH is more sensitive in the detection of trisomy 8 than CC, and FISH displays its superiority in the detection of small clone. |
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| Keywords: | Acute lymphoblastic leukemia Chromosome 8 Cytogenetics Fluorescence in situ hybridization |
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